Use of phages against antibiotic-resistant Staphylococcus aureus isolated from bovine mastitis
Use of phages against antibiotic-resistant Staphylococcus aureus isolated from bovine mastitis
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DOI：
10.2527/jas.2012-5884
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发表时间：
2013-08-01
影响因子：
3.3
通讯作者：
De Paula, S. O.
中科院分区：
文献类型：
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作者：
Dias, R. S.;Eller, M. R.;De Paula, S. O.
牛乳腺炎是世界范围内奶牛的主要疾病，造成巨大的经济损失。在引起此病的几种微生物中，金黄色葡萄球菌最为常见。虽然抗生素治疗仍然是治疗牛乳腺炎最广泛使用的方法，但由于牛奶中存在抗生素残留，因此需要替代治疗方法，这是一个日益关注的问题，因为它干扰了牛奶衍生物的生产和耐药菌株的选择。使用噬菌体作为控制病原体的工具是抗生素治疗的一种替代治疗方法。在这项工作中，为了获得具有噬菌体治疗乳腺炎潜力的噬菌体，我们从乳腺炎阳性奶牛的牛奶中分离并鉴定了细菌。巴西Zona da Mata Mineira的中小型养殖场总共有19%的牛乳腺炎呈阳性，葡萄球菌属细菌是最普遍的病原体。大多数金黄色葡萄球菌分离株对青霉素和氨苄西林耐药。同时，我们分离了10个能够感染这些金黄色葡萄球菌分离物的噬菌体。我们确定这些噬菌体含有长度约为175 kb的DNA基因组，蛋白质谱表明存在4种主要蛋白质。电镜显示噬菌体呈尾状，属于肌病毒科。这些分离物在噬菌体治疗中表现出有趣的特征，如高裂解潜力、广泛的宿主和热稳定性，所有这些都有利于它们在该领域的应用。
Bovine mastitis is the primary disease of dairy cattle worldwide and it causes large economic losses. Among several microorganisms that are the causative agents of this disease, Staphylococcus aureus is the most prevalent. Although antibiotic therapy is still the most widely used procedure for the treatment of bovine mastitis, alternative means of treatment are necessary due to the presence of antibiotic residues in milk, which is a growing concern because of its interference with the production of milk derivatives and the selection of resistant bacterial strains. The use of bacteriophages as a tool for the control of pathogens is an alternative treatment to antibiotic therapy. In this work, to obtain phages with the potential for use in phage therapy as a treatment for mastitis, we isolated and identified the bacteria from the milk of mastitis-positive cows. A total of 19% of the animals from small and medium farms of the Zona da Mata Mineira, Brazil, was positive for bovine mastitis, and bacteria of the genus Staphylococcus were the most prevalent pathogens. The majority of the S. aureus isolates tested was resistant to penicillin and ampicillin. In parallel, we isolated 10 bacteriophages able to infect some of these S. aureus isolates. We determined that these phages contained DNA genomes of approximately 175 kb in length, and the protein profiles indicated the presence of 4 major proteins. Electron microscopy revealed that the phages are caudate and belong to the Myoviridae family. The isolates exhibited interesting features for their use in phage therapy such as a high lytic potential, a wide range of hosts, and thermostability, all of which favor their use in the field.