RNA-catalyzed amino acid activation
RNA-catalyzed amino acid activation
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DOI：
10.1021/bi010710x
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发表时间：
2001-06-19
期刊：
影响因子：
2.9
通讯作者：
Yarus, M
中科院分区：
文献类型：
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作者：
Kumar, RK;Yarus, M
我们选择的RNA执行一种新的化学反应，激活氨基酸，平行混合磷酸酐合成的蛋白质氨基酰基转移RNA合成酶。注意不稳定反应产物的回收显然对这种选择至关重要。表征最好的RNA是KK13，它只需要Ca2+就能反应，在低pH下k - m = 50 mM, k(cat) = 1.1 min（-1）活化亮氨酸。结合之前rna催化的氨基酰基rna合成、肽键形成和rna编码，这些氨基酸激活rna完成了一个实验证明，蛋白质生物合成的四个基本反应可以由rna介导。因此，翻译在RNA世界的出现得到了支持。
We have selected RNAs that perform a new reaction that chemically activates amino acids, paralleling mixed phosphate anhydride synthesis by protein aminoacyl-transfer RNA synthetases. Care with recovery of the unstable reaction product was apparently essential to this selection. The best characterized RNA, KK13, requires only Ca2+ for reaction and is optimally active at low pH with K-M = 50 mM and k(cat) = 1.1 min(-1) for activation of leucine. In conjunction with previous RNA-catalyzed aminoacyl-RNA synthesis, peptide bond formation, and RNA-based coding, these amino acid-activating RNAs complete an experimental demonstration that the four fundamental reactions of protein biosynthesis can be RNA-mediated. The appearance of translation in an RNA world is therefore supported.