Establishment of a set of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), and evidence of their varying levels of virulence
Establishment of a set of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), and evidence of their varying levels of virulence
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DOI：
10.1007/s13355-012-0124-8
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发表时间：
2012-07
影响因子：
1.3
通讯作者：
R. Shinya;Y. Takeuchi;Keita Ichimura;S. Takemoto;K. Futai
中科院分区：
文献类型：
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作者：
R. Shinya;Y. Takeuchi;Keita Ichimura;S. Takemoto;K. Futai
松材线虫病是由松材线虫松材线虫引起的世界性病害。PWD的发病机制仍然存在争议，部分原因可能是缺乏通用的B物质。具有很高的遗传纯度的嗜木菌。固有的高度遗传多样性。对于执行正向遗传学和其他控制它们的分子方法来说，嗜木质单胞菌/种群必须是一个致命的障碍。我们进行了一系列常规分离株的全同胞交配。建立一套近交系。利用DNA分子标记检测了它们的遗传多样性和致病力、繁殖力等生物学特性。因此，新建立的菌株产生了比传统菌株更高的遗传纯度，并显示出不同的毒力，尽管有共同的祖先。本研究的意义不仅在于建立了一套自交系。嗜木素不仅证明了它们的纯度，而且还证明了通过简单连续的全同胞交配可以获得与强毒株具有相似基因的无毒株(S)。这项技术是阐明PWD的发病机制(S)的最有力的工具之一。
Pine wilt disease (PWD) caused by the pine wood nematode,Bursaphelenchus xylophilus(Steiner and Buhrer) Nickle, has become a worldwide problem. The pathogenic mechanism of PWD continues to remain controversial, which in part may be attributed to the lack of universal materials ofB. xylophiluswith a high genetic purity. The intrinsic high genetic diversity inB. xylophilusisolates/populations must be a fatal obstacle for performing forward genetics and other molecular approaches to controlling them. We conducted a series of successive full-sib mating of conventional isolates ofB. xylophilusto establish a set of inbred strains. Using DNA markers, we also determined their genetic diversity and biological characteristics, such as virulence and reproductive ability. Consequently, the newly established strains yielded a higher genetic purity than the conventional isolates and showed varying virulence despite sharing a common ancestor. The significance of this study lies not only in establishing a set of inbred strains ofB. xylophiluswith the certification of their purity but also in demonstrating that avirulent strain(s) with a genotype similar to the virulent strains can be obtained by simple successive full-sib mating. This technique is one of the most powerful tools for elucidating the pathogenic mechanism(s) of PWD.