Activity of human Δ5 and Δ6 desaturases on multiple n-3 and n-6 polyunsaturated fatty acids
Activity of human Δ5 and Δ6 desaturases on multiple n-3 and n-6 polyunsaturated fatty acids
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DOI：
10.1016/s0014-5793(01)03135-0
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发表时间：
2001-11-30
期刊：
影响因子：
3.5
通讯作者：
Winther, MD
中科院分区：
文献类型：
--
作者：
de Antueno, RJ;Knickle, LC;Winther, MD
用酵母共表达人类延长酶和去饱和酶基因来研究相同的去饱和酶基因是否编码一种能够使碳链长度相同或不同的n-3和n-6脂肪酸去饱和的酶。结果清楚地表明，单个人Delta5去饱和酶在20:3n-6和20:4n-3上具有活性。内源性Delta6去饱和酶底物是通过向酵母提供放射性标记的20:4n-6或20:5n-3，通过两次连续延伸，分别产生24:4n-6和24:5n-3。总的来说，我们的数据表明单个人类Delta6去饱和酶在18:2n-6、18:3n-3、24:4n-6和24:5n-3有活性。(C) 2001年由Elsevier Science B.V.代表欧洲生化学会联合会出版。
Yeast co-expressing human elongase and desaturase genes were used to investigate whether the same desaturase gene encodes an enzyme able to desaturate n-3 and n-6 fatty acids with the same or different carbon chain length. The results clearly demonstrated that a single human Delta5 desaturase is active on 20:3n-6 and 20:4n-3. Endogenous Delta6 desaturase substrates were generated by providing to the yeast radiolabelled 20:4n-6 or 20:5n-3 which, through two sequential elongations, produced 24:4n-6 and 24:5n-3, respectively. Overall, our data suggest that a single human Delta6 desaturase is active on 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.