Pulmonary and neurological health effects associated with exposure to representative composite manufacturing emissions and corresponding alterations in circulating metabolite profiles
Pulmonary and neurological health effects associated with exposure to representative composite manufacturing emissions and corresponding alterations in circulating metabolite profiles
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与接触代表性复合制造排放物以及循环代谢物谱的相应变化相关的肺和神经健康影响
DOI：
10.1093/toxsci/kfad029
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发表时间：
2023
影响因子：
3.8
通讯作者：
Shannahan, Jonathan H
中科院分区：
文献类型：
--
作者：
Xia, Li;Noh, Yoorae;Whelton, Andrew J;Boor, Brandon E;Cooper, Bruce;Lichti, Nathanael I;Park, Jae Hong;Shannahan, Jonathan H
现场固化管道（CIPP）技术越来越多地被用于修复老化和损坏的管道，然而，存在与排放物的公共健康危害相关的担忧。CIPP安装涉及在工地制造新的塑料复合管，并包括多个可变组件，包括树脂材料，固化方法和操作条件。我们假设苯乙烯基复合材料制造排放物（CME）将诱导更大的肺部炎症反应和氧化应激，以及神经毒性相比，nonstyrene CME。此外，这些CME毒理学反应将具有性别和时间依赖性。为了验证这一假设，使用实验室固化室产生代表性的CME，并使用热解吸-气相色谱-质谱和光电离检测器进行表征。苯乙烯被释放期间停留，等温固化，和冷却阶段的过程中，并在冷却阶段达到峰值。雄性和雌性C57 BL 6/J小鼠用于检查暴露于空气（对照）、非苯乙烯-CME或苯乙烯-CME后1天和7天肺反应和神经毒性的变化。暴露于苯乙烯-CME的小鼠血清苯乙烯代谢产物增加。代谢和脂质分析显示与CIPP排放相关的改变是树脂，时间和性别依赖性的。暴露于苯乙烯-CME导致两种性别的淋巴细胞流入。炎症和氧化应激标志物的表达，包括Tnf α、Vcam 1、Ccl 2、Cxcl 2、IL 6、Cxcl 1、TGFβ1、Tgmt 2和Hmox 1，在暴露于放射物后显示出变化。毒性的肺和神经学标志物的这些变化取决于树脂类型、性别和时间。总的来说，本研究证明了代表性CME的树脂特异性差异和毒性终点的改变，这可能有助于更安全地利用复合材料制造工艺。
Cured-in-place pipe (CIPP) technology is increasingly being utilized to repair aging and damaged pipes, however, there are concerns associated with the public health hazards of emissions. CIPP installation involves the manufacture of a new plastic composite pipe at the worksite and includes multiple variable components including resin material, curing methods, and operational conditions. We hypothesize styrene-based composite manufacturing emissions (CMEs) will induce greater pulmonary inflammatory responses and oxidative stress, as well as neurological toxicity compared with nonstyrene CMEs. Further, these CME-toxicological responses will be sex- and time-dependent. To test the hypothesis, representative CMEs were generated using a laboratory curing chamber and characterized using thermal desorption-gas chromatography-mass spectrometry and photoionization detector. Styrene was released during staying, isothermal curing, and cooling phases of the process and peaked during the cooling phase. Male and female C57BL6/J mice were utilized to examine alterations in pulmonary responses and neurotoxicity 1 day and 7 days following exposure to air (controls), nonstyrene-CMEs, or styrene-CMEs. Serum styrene metabolites were increased in mice exposed to styrene-CMEs. Metabolic and lipid profiling revealed alterations related to CIPP emissions that were resin-, time-, and sex-dependent. Exposure to styrene-CMEs resulted in an influx of lymphocytes in both sexes. Expression of inflammatory and oxidative stress markers, includingTnfα, Vcam1, Ccl2, Cxcl2,Il6,Cxcl1,Tgfβ1,Tgmt2, andHmox1, displayed alterations following exposure to emissions. These changes in pulmonary and neurological markers of toxicity were dependent on resin type, sex, and time. Overall, this study demonstrates resin-specific differences in representative CMEs and alterations in toxicity endpoints, which can potentially inform safer utilization of composite manufacturing processes.