High-level transgene expression in neurons by lentivirus with Tet-Off system
High-level transgene expression in neurons by lentivirus with Tet-Off system
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DOI：
10.1016/j.neures.2008.10.010
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发表时间：
2009-02-01
影响因子：
2.9
通讯作者：
Kaneko, Takeshi
中科院分区：
文献类型：
--
作者：
Hioki, Hiroyuki;Kuramoto, Eriko;Kaneko, Takeshi
我们利用Tet-Off系统开发了新型慢病毒载体，并成功实现了体内高水平和神经元特异性的基因转导。在将病毒注射到大鼠新纹状体中一周后，GFP表达几乎完全是神经元特异性的，并且比常规慢病毒载体的表达高约40倍。高转录活性和神经元特异性持续长达8周。此外，通过向GFP添加质膜靶向信号，感染神经元的神经元过程被有效地可视化。这些结果表明，本方法是有价值的强大的基因转导和清晰的可视化神经元在体内。(C)2008年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
We developed novel lentiviral vectors by using "Tet-Off system" and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were efficiently visualized by adding a plasma membrane-targeting signal to GFP. These results suggest that the present method is valuable for strong gene transduction and clear visualization of neurons in vivo. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.