Acetylcholine active transport by rat brain synaptic vesicles.
Acetylcholine active transport by rat brain synaptic vesicles.
复制标题
大鼠脑突触小泡的乙酰胆碱主动转运。
DOI：
10.1097/00001756-199403000-00009
复制
发表时间：
1994
期刊：
影响因子：
1.7
通讯作者：
Parsons,SM
中科院分区：
文献类型：
--
作者：
Haigh,JR;Noremberg,K;Parsons,SM
在从大鼠脑中分离的突触囊泡组分中研究了乙酰胆碱的摄取。在MgATP存在下，低渗处理的P3囊泡摄取乙酰胆碱（ACh），低温、铵离子、质子载体羰基氰对三氟甲氧基苯腙（FCCP）和液泡H+-ATP酶特异性抑制剂巴弗洛霉素A1均能抑制这种摄取。摄取也受到与ACh转运蛋白变构结合的药物的抑制，即vesamicol（IC 50值为170 nM）和4-aminobenzovesamicol（IC 50值为25 nM）。KM和V最大值ACh主动运输估计为5 mM和4 nmol min-1 mg-1的胆碱能囊泡，分别。乙酰胆碱的主动运输由突触囊泡部分纯化的大脑介导的vesamicol敏感的转运蛋白，并依赖于质子梯度产生的囊泡H+-ATP酶。
UPTAKE of acetylcholine was studied in a synaptic vesicle fraction isolated from rat brain. Hyposmotically treated P3 vesicles took up acetylcholine (ACh) in the presence of MgATP, and the uptake was inhibited by low temperature, ammonium ions, the protonophore carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP) and bafilomycin A 1, a specific inhibitor of the vacuolar H+-ATPase. Uptake was also inhibited by drugs that bind allosterically to the ACh transporter, namely vesamicol (IC 50 value of 170 nM) and 4-aminobenzovesamicol (IC 50 value of 25 nM). K M and V max values for ACh active transport were estimated to be 5 mM and 4 nmol min-1 mg-1 of cholinergic vesicles, respectively. Active transport of ACh by synaptic vesicles partially purified from brain is mediated by a vesamicol-sensitive transporter and is dependent on a proton gradient generated by the vesicular H+-ATPase.