Differential expression of lipid metabolism-related genes and miRNAs in Ctenopharyngodon idella liver in relation to fatty liver induced by high non-protein energy diets
Differential expression of lipid metabolism-related genes and miRNAs in Ctenopharyngodon idella liver in relation to fatty liver induced by high non-protein energy diets
复制标题
草鱼肝脏脂质代谢相关基因和miRNA的差异表达与高非蛋白能量饮食诱导脂肪肝的关系
DOI：
10.1111/are.13228
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发表时间：
2017
影响因子：
2
通讯作者：
Nie Guo-xing
中科院分区：
文献类型：
--
作者：
Wang Jun-li;Lu Rong-hua;Sun Jun-jun;Xie Di-zhi;Yang Feng;Nie Guo-xing
为探讨高非蛋白能量饲料诱导草鱼脂肪肝形成的机制，以草鱼幼鱼为试验对象，分别饲喂基础饲料和高能量饲料9周。实验组喂食高能量饮食，包括高脂饮食（H-LIP）、高碳水化合物饮食（H-CHO）和高脂和碳水化合物饮食（H-CL）。对照组饲喂基础日粮。饲养试验结束时检测各组大鼠的生长性能、肝脏脂肪蓄积、血清生化指标以及脂代谢相关基因（SREBP-1、PPARγ、FAS、ACC 1和LPL）和miRNAs（miR-33、miR-122和miR-370）的表达水平。四组间的生长速度和饲料效率差异不显著。然而，高脂和高碳水化合物饮食诱导了肠系膜和肝脏脂肪含量以及肝脏中脂滴的显著增加。对照组与实验组血清AST/ALT、GLB、TG、TP等生化指标及肝脏脂肪酸组成差异显著。高能辐射可上调SREBP-1、PPARγ、FAS、ACC-1和LPL的表达，下调CPT-1的表达。此外，3种高能处理组肝脏中miR-33、miR-122和miR-370的表达水平均高于对照组（P <0.05）。结果提示，高非蛋白能量饲料诱导草鱼脂肪肝的形成可能与脂代谢相关基因和miRNAs的改变有关。
To explore the mechanism of fatty liver formation induced by high non‐protein energy diets in grass carp (Ctenopharyngodon idella), basal diet and high‐energy diets were fed to juvenile grass carp for 9 weeks. The experimental groups fed on high‐energy diets which included a high‐lipid diet (H‐LIP), a high‐carbohydrate diet (H‐CHO) and a high‐lipid and carbohydrate diet (H‐CL). The control group fed on basal diet. Growth performance, liver fat accumulation, serum biochemical indexes and the expression levels of lipid metabolism‐related genes (SREBP‐1,PPARγ,FAS,ACC1, andLPL) and miRNAs (miR‐33, miR‐122, and miR‐370) were examined at the end of the feeding trial. There were no significant differences in growth rate and feed efficiency among the four groups. However, significant increase in mesenteric and liver fat contents, and lipid droplets in the liver was induced by high‐lipid and high‐carbohydrate diets. There were significant differences in serum biochemical indicators such as AST/ALT, GLB, TG and TP, and liver fatty acid composition between the control and experimental groups. The expression levels ofSREBP‐1,PPARγ,FAS,ACC1andLPLwere upregulated, whileCPT‐1was downregulated with the high‐energy treatments. Additionally, the expression levels of miR‐33, miR‐122 and miR‐370 in the liver were higher in the three high‐energy treatments than those in the control (P <0.05). The results suggest that modifications of lipid metabolism‐related genes and miRNAs may be involved in fatty liver formation induced by high non‐protein energy diets in grass carp.