EXPRESSION PATTERNS OF LORICRIN IN VARIOUS SPECIES AND TISSUES
EXPRESSION PATTERNS OF LORICRIN IN VARIOUS SPECIES AND TISSUES
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DOI：
10.1111/j.1432-0436.1993.tb00656.x
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发表时间：
1993-08-01
期刊：
影响因子：
2.9
通讯作者：
ROOP, DR
中科院分区：
文献类型：
--
作者：
HOHL, D;OLANO, BR;ROOP, DR
在这项研究中，我们利用免疫组织化学、免疫印迹和Northern blotting分析了氯化蛋白在不同物种和组织中的表达模式。Loricrin是一种富含甘氨酸、丝氨酸和半胱氨酸的蛋白质，在正常小鼠和人类表皮的颗粒层中表达的很晚。在分化后期，氯化蛋白作为主要成分通过形成N(Epsilon)-(γ-谷氨酰基)赖氨酸异肽键而交联化进入角化细胞被膜。这一过程既可直接发生，也可通过L-角化透明质颗粒在小鼠表皮和人肢端汗液中的中间蓄积而发生。Loricrin在所有哺乳动物中都得到了鉴定，其高度保守的羧基末端序列在啮齿动物、兔子和奶牛中显示出约60 kDa的电迁移率，在羊肉和人的十二烷基硫酸钠聚丙烯酰胺凝胶电泳法上显示出约35 kDa的电迁移率。Loricrin在所有哺乳动物的口腔、食道和前胃黏膜，维生素A缺乏的仓鼠的气管鳞状化生和去卵巢大鼠的雌激素诱导的阴道鳞状上皮的颗粒层中都有表达。Loricrin也在少数角化旁上皮细胞中表达，如BBN、[N-butyl-N-(4-hydroxybutyl)nitrosamine]-induced、小鼠膀胱癌和高等哺乳动物口腔和单个阴道上皮细胞的限制性亚群。我们的结果进一步证明，啮齿类动物和高等哺乳动物上消化道内上皮细胞的鳞状分化程序明显不同。此外，我们还注意到角化细胞膜的另一种主要前体蛋白--人氯化蛋白和总蛋白的不同分布模式。
In this study we analyzed the expression patterns of loricrin in various species and tissues using immunohistochemistry, immunoblotting and Northern blots. Loricrin is a glycine-, serine- and cysteine-rich protein expressed very late in epidermal differentiation in the granular layers of normal mouse and human epidermis. Later on in differentiation, loricrin becomes cross-linked as a major component into the cornified cell envelope by the formation of N(epsilon)-(gamma-glutamyl)lysine isopeptide bonds. This process either occurs directly or by the intermediate accumulation in L-keratohyaline granules of mouse epidermis and human acrosyringia. Loricrin was identified in all mammalian species analyzed by virtue of its highly conserved carboxy-terminal sequences revealing an electric mobility of approximately 60 kDa in rodents, rabbit and cow and of approximately 35 kDa in lamb and human on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Loricrin is expressed in the granular layer of all mammalian orthokeratinizing epithelia tested including oral, esophageal and fore-stomach mucosa of rodents, tracheal squamous metaplasia of vitamin A deficient hamster and estrogen induced squamous vaginal epithelium of ovary ectomized rats. Loricrin is also expressed in a few parakeratinizing epithelia such as BBN [N-butyl-N-(4-hydroxybutyl)nitrosamine]-induced murine bladder carcinoma and a restricted subset of oral and single vaginal epithelial cells in higher mammals. Our results provide further evidence that the program of squamous differentiation in internal epithelia of the upper alimentary tract in rodents and higher mammals differ remarkably. In addition, we also have noted the distinct distribution patterns of human loricrin and involucrin, another major precursor protein of the cornified cell envelope.