Periostin is a negative regulator of mineralization in the dental pulp tissue
Periostin is a negative regulator of mineralization in the dental pulp tissue
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DOI：
10.1007/s10266-014-0152-7
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发表时间：
2015-05
期刊：
影响因子：
2.5
通讯作者：
Meng Zhou;N. Kawashima;Noriyuki Suzuk;Mioko Yamamoto;K. Ohnishi;K. Katsube;H. Tanabe;A. Kudo;M. Saito;H. Suda
中科院分区：
文献类型：
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作者：
Meng Zhou;N. Kawashima;Noriyuki Suzuk;Mioko Yamamoto;K. Ohnishi;K. Katsube;H. Tanabe;A. Kudo;M. Saito;H. Suda
牙髓组织包裹在硬组织中，并被硬组织形成细胞包围，但本身仍处于非矿化状态，这表明存在阻止牙髓矿化的调节机制。本研究旨在揭示成骨细胞分化所必需的骨膜蛋白（Postn）对成牙本质细胞分化/矿化的调节功能。我们评估了Postn过表达和RNAi介导的小鼠牙乳头细胞（MDP）抑制对成牙本质细胞标志物和Notch信号分子表达以及矿化结节形成的影响。用免疫组织化学方法观察Postn在正常磨牙和洞制备磨牙牙髓组织中的定位。MDP中Postn的强制过表达诱导成牙本质细胞标记物和体外矿化的下调。相反，MDP中Postn mRNA的沉默诱导成牙本质细胞标志物和ALP活性的上调。Postn的上调和下调分别导致Notch信号分子的表达增加和减少。Postn在正常牙髓中的表达是最小的，但在洞制备后1天，在磨牙的整个牙髓组织中迅速和全面地增加，此后减少。这些结果表明，Postn可能是成牙本质细胞分化/矿化的负调控因子，并且可能通过Notch信号发挥其作用。
The dental pulp tissue is encased in hard tissue and surrounded by hard tissue-forming cells, but remains in a non-mineralized state itself, suggesting the presence of regulatory mechanisms precluding pulp mineralization. This study aimed to reveal the regulatory function of periostin (Postn), which is essential for osteoblast differentiation, for odontoblast differentiation/mineralization. We evaluated the effects of Postn overexpression and RNAi-mediated suppression in mouse dental papilla cells (MDPs) on the expression of odontoblastic markers and Notch signaling molecules, and on the formation of mineralized nodules. Localization of Postn in the dental pulp tissue of normal and cavity-prepared molars was observed immunohistologically. Enforced overexpression of Postn in MDPs induced down-regulation of odontoblastic markers and in vitro mineralization. Conversely, silencing of Postn mRNA in MDPs induced up-regulation of odontoblastic markers and ALP activity. Up- and down-regulation of Postn caused increased and decreased expression, respectively, of Notch signaling molecules. Postn expression was minimal in normal dental pulp, but was rapidly and globally increased in the whole pulp tissue of molar teeth at 1 day after cavity preparation, decreasing thereafter. These results indicate that Postn may be a negative regulator of odontoblast differentiation/mineralization, and that may exert its actions via Notch signals.