Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes
Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes
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DOI：
10.1126/science.1081453
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发表时间：
2003-02-21
期刊：
影响因子：
56.9
通讯作者：
Crisanti, A
中科院分区：
文献类型：
--
作者：
Catteruccia, F;Godfray, HCJ;Crisanti, A
蚊子的基因改造为控制疟疾提供了令人兴奋的可能性，但成功与否将取决于改造如何影响改造昆虫的适应性。外源基因的表达、其插入引起的突变以及建立转化系时的近交都可能导致适合度的降低。在笼内实验中，研究了影响适合度的因素，用四个品系的转基因斯氏按蚊，一种人类疟疾的媒介物种。结果表明，在四个品系中的至少三个品系中引入的转基因的直接成本，以及涉及制备转基因纯合子的近交的明显成本。
Genetic modification of mosquitoes offers exciting possibilities for controlling malaria, but success will depend on how transformation affects the fitness of modified insects. The expression of an exogenous gene, the mutations caused by its insertion, and inbreeding while transformed lines are established can all lead to reductions in fitness. Factors influencing fitness were investigated in cage experiments with four lines of transgenic Anopheles stephensi, a vector species of human malaria. The results indicate direct costs of the introduced transgene in at least three out of the four lines, as well as an apparent cost of the inbreeding involved in making transgenic homozygotes.