Interaction trap/two-hybrid system to identify interacting proteins.
Interaction trap/two-hybrid system to identify interacting proteins.
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DOI：
10.1002/0471140864.ps1902s57
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Brent, Roger
中科院分区：
文献类型：
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作者：
Golemis, Erica A;Serebriiskii, Ilya;Brent, Roger
酵母双杂交方法（或相互作用陷阱）是一种检测蛋白质相互作用的强大技术。该程序使用酵母中的双报告系统的转录激活来进行，以鉴定感兴趣的蛋白质（诱饵蛋白）和用于相互作用的候选蛋白质之间的相互作用。该方法可用于筛选与诱饵蛋白相互作用的蛋白质文库，或用于测试基于先前证据预期相互作用的蛋白质之间的关联。相互作用交配促进了具有多种诱饵蛋白的文库的筛选。
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.