DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test.
DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test.
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发表时间：
2014-09
影响因子：
1.4
通讯作者：
Kui Li;Ji-wei Liu;Zhi-Chuan Zhu;Hong-Tao Wang;Y. Zu;Yong-jie Liu;Yanhong Yang;Z. Xiong;Xu Shen;Rui Chen;Jing Zheng;Zelan Hu
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文献类型：
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作者：
Kui Li;Ji-wei Liu;Zhi-Chuan Zhu;Hong-Tao Wang;Y. Zu;Yong-jie Liu;Yanhong Yang;Z. Xiong;Xu Shen;Rui Chen;Jing Zheng;Zelan Hu
DSTYK（Dual serine/threonine and tyrosine protein kinase）是一种具有独特结构特征的双丝氨酸/苏氨酸和酪氨酸蛋白激酶。由于DSTYK在脑内大部分区域的高表达，推测其在脑内可能发挥重要作用。在本研究中，DSTYK基因敲除（KO）小鼠与切除的C-末端的DSTYK包括激酶结构域的产生，以研究DSTYK的生理功能。与野生型小鼠相比，DSTYK KO小鼠是可生育的，并且通过尼氏染色显示没有显著的形态缺陷。旷场试验和转棒试验表明，DSTYK纯合子KO小鼠与DSTYK杂合子KO小鼠的基本运动能力和平衡能力无明显差异。而在水迷宫实验中，与DSTYK杂合子KO小鼠相比，DSTYK纯合子KO小鼠的学习记忆能力明显下降。
DSTYK (Dual serine/threonine and tyrosine protein kinase) is a putative dual Ser/Thr and Tyr protein kinase with unique structural features. It is proposed that DSTYK may play important roles in brain because of its high expression in most brain areas. In the present study, a DSTYK knockout (KO) mouse line with the ablation of C-terminal of DSTYK including the kinase domain was generated to study the physiological function of DSTYK. The DSTYK KO mice are fertile and have no significant morphological defects revealed by Nissl staining compared with wildtype mice. Open field test and rotarod test showed there is no obvious difference in basic motor and balance capacity between the DSTYK homozygous KO mice and DSTYK heterozygous KO mice. In water maze test, however, the DSTYK homozygous KO mice show impaired capabilities of learning and memory compared with the DSTYK heterozygous KO mice.