Characterization of plant circadian rhythms by employing Arabidopsis cultured cells with bioluminescence reporters
Characterization of plant circadian rhythms by employing Arabidopsis cultured cells with bioluminescence reporters
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DOI：
10.1093/pcp/pch003
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Mizuno, T
中科院分区：
文献类型：
--
作者：
Nakamichi, N;Ito, S;Mizuno, T
最近的深入研究已经开始揭示拟南芥植物生物钟的分子机制。在这些先前的研究过程中，最强大的技术，优雅地采用了一种实时生物发光监测系统，监测携带荧光素酶（LUC）融合转基因的完整植物的昼夜节律。我们之前证明，拟南芥培养的细胞也保留了产生昼夜节律的能力，至少在一定程度上是这样。为了进一步完善用于昼夜节律研究的培养细胞系统，本研究采用生物发光监测系统，建立了携带适当报告基因CCA1::LUC和APRR1::LUC的细胞系，其中CCA1 （circadian CLOCK-ASSOCIATED1）和APRR1（或TOC1）（拟南芥伪响应调节因子s1或CAB表达时序1）被认为是中心振荡器的组成部分。我们报告的结果一致地支持这样的观点，即已建立的细胞系，配备了这样的生物发光报告者，可能为我们提供了一种有利的手段来表征植物昼夜节律。
Recent intensive studies have begun to shed light on the molecular mechanisms underlying the plant circadian clock in Arabidopsis thaliana. During the course of these previous studies, the most powerful technique, elegantly adopted, was a real-time bioluminescence monitoring system of circadian rhythms in intact plants carrying a luciferase (LUC) fusion transgene. We previously demonstrated that Arabidopsis cultured cells also retain an ability to generate circadian rhythms, at least partly. To further improve the cultured cell system for studies on circadian rhythms, here we adopted a bioluminescence monitoring system by establishing the cell lines carrying appropriate reporter genes, namely, CCA1::LUC and APRR1::LUC, with which CCA1 (CIRCADIAN CLOCK-ASSOCIATED1) and APRR1 (or TOC1) (ARABIDOPSIS PSEUDO-RESPONSE REGULATORS1 or TIMING OF CAB EXPRESSION1) are believed to be the components of the central oscillator. We report the results that consistently supported the view that the established cell lines, equipped with such bioluminescence reporters, might provide us with an advantageous means to characterize the plant cireadian clock.