A prolonged cold treatment-induced cytochrome P450 gene from Arabidopsis thaliana
A prolonged cold treatment-induced cytochrome P450 gene from Arabidopsis thaliana
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DOI：
10.1046/j.1365-3040.1999.00444.x
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发表时间：
1999-07-01
影响因子：
7.3
通讯作者：
Dennis, ES
中科院分区：
文献类型：
--
作者：
Bilodeau, P;Udvardi, MK;Dennis, ES
据报道，来自拟南芥 (CYP83A1) 的全长细胞色素 P450 (cyt P450) cDNA 克隆在长期低温处理后在茎尖出现 2-4 倍的转录诱导，CYP83A1 似乎由单拷贝基因编码。该基因包含一个内含子，其位置与其他 A 类 P450 基因中发现的位置相同。在启动子区域鉴定了参与苯丙素/类黄酮生物合成基因（SBF-1、MYB Ph3 和 P-MYB）调节的推定顺式作用元件。该编码区在酵母中具有结合一氧化碳和四环素的功能，表明 CYP83A1 以其天然状态产生，使其能够与这两种细胞色素 P450 抑制剂正确相互作用。然而，在赤霉素或苯丙素生物合成的 P450 依赖性反应中检测不到活性。表达有义和反义转录物的转基因拟南芥植物在有或没有春化的情况下均未表现出任何异常或改变的开花时间。
The characterization of a full length cytochrome P450 (cyt P450) cDNA clone from Arabidopsis thaliana (CYP83A1) which showed a 2-4-fold transcriptional induction in the shoot apex following a prolonged low temperature treatment is reported, CYP83A1 appears to be encoded by a single copy gene. The gene contains one intron in a position identical to that found in other class A P450 genes. Putative cis-acting elements implicated in the regulation of phenylpropanoid/flavonoid biosynthetic genes (SBF-1, MYB Ph3, and P-MYB) were identified in the promoter region. The coding region was functional in yeast in binding carbon monoxide and tetcyclacis, suggesting that CYP83A1 was produced in its native state enabling it to interact properly with these two cyt P450 inhibitors. However, no activity could be detected when assayed in P450-dependent reactions of gibberellin or phenylpropanoid biosynthesis. Transgenic Arabidopsis plants expressing sense and antisense transcripts did not show any abnormalities or altered flowering time with or without vernalization.