CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis
CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis
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DOI：
10.1073/pnas.0705147104
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发表时间：
2007-12-04
影响因子：
11.1
通讯作者：
Shibuya, Naoto
中科院分区：
文献类型：
--
作者：
Miya, Ayako;Albert, Premkumar;Shibuya, Naoto
几丁质是真菌细胞壁的主要成分，在植物和动物的先天免疫系统中作为微生物相关分子模式（MAMP）检测各种潜在病原体。我们最近发现，几丁质激发子结合蛋白（CEBiP）是一种具有LysM基序的质膜糖蛋白，在水稻中作为几丁质激发子的细胞表面受体。预测的CEBiP结构不包含任何胞内结构域，这表明需要一个额外的成分才能通过质膜进入细胞质。在这里，我们发现了一种受体样激酶，称为CERK1，它对拟南芥中几丁质激发子信号传导至关重要。CERK1的KO突变体完全失去了对几丁质激发子的反应能力，包括MAPK激活、活性氧产生和基因表达。KO突变体对一种不相容的真菌——十字花科真菌的抗病能力部分受损。与WT CERK1基因的互补表明，CERK1突变是导致突变表型的原因。CERK1是一种质膜蛋白，在细胞外结构域含有3个LysM基元，在细胞内含有一个Ser/Thr激酶结构域，具有自磷酸化/髓鞘碱性蛋白激酶活性，这表明CERK1在植物真菌MAMP感知中起关键作用。
Chitin is a major component of fungal cell walls and serves as a microbe-associated molecular pattern (MAMP) for the detection of various potential pathogens in innate immune systems of both plants and animals. We recently showed that chitin elicitor-binding protein (CEBiP), plasma membrane glycoprotein with LysM motifs, functions as a cell surface receptor for chitin elicitor in rice. The predicted structure of CEBiP does not contain any intracellular domains, suggesting that an additional component(s) is required for signaling through the plasma membrane into the cytoplasm. Here, we identified a receptor-like kinase, designated CERK1, which is essential for chitin elicitor signaling in Arabidopsis. The KO mutants for CERK1 completely lost the ability to respond to the chitin elicitor, including MAPK activation, reactive oxygen species generation, and gene expression. Disease resistance of the KO mutant against an incompatible fungus, Alternaria brassicicola, was partly impaired. Complementation with the WT CERK1 gene showed cerk1 mutations were responsible for the mutant phenotypes. CERK1 is a plasma membrane protein containing three LysM motifs in the extracellular domain and an intracellular Ser/Thr kinase domain with autophosphorylation/myelin basic protein kinase activity, suggesting that CERK1 plays a critical role in fungal MAMP perception in plants.