Structure of the Freeze‐Fractured Sarcolemma in the Normal and Anoxic Rabbit Myocardium
Structure of the Freeze‐Fractured Sarcolemma in the Normal and Anoxic Rabbit Myocardium
复制标题
正常和缺氧兔心肌冻裂肌膜的结构
DOI：
10.1161/01.res.47.1.131
复制
发表时间：
1980
影响因子：
20.1
通讯作者：
E. Rau
中科院分区：
文献类型：
--
作者：
J. Frank;S. Beydler;M. Kreman;E. Rau
本研究采用冻裂技术对正常和严重缺氧兔心脏肌膜的超微结构进行了研究。严重缺氧和随后的再氧合导致膜P面膜内颗粒（IMP）显著减少（31%），E面膜内颗粒（IMP）显著减少25%。P面IMP严重聚集。密度的降低和IMP的重新分布表明肌膜上蛋白结构严重改变。此外，小泡的颈部打开，小泡在膜平面上变平。随着再氧化，许多细胞破裂。在膜上（可能是肌浆网或溶酶体起源）可见胞质囊泡的球形突起，再氧化后也可见破裂。当葡萄糖存在于灌注液中时，它对这些结构缺陷提供了一些保护。我们认为，在严重缺氧的情况下，肌外膜的破碎或空洞与本研究中报道的结构变化直接相关。CircRes 47:131- 143,1980
The purpose of this study was to examine the ultrastructure of the sarcolemma in the normal and severely anoxic rabbit heart with the technique of freeze-fracture. Severe anoxia and subsequent reoxygenation cause a significant decrease (31%) in intramembranous particles (IMP) in the P face of the membrane and a 25% decrease in the E face. P face IMP's are severely aggregated. The decrease in density and the redistribution of IMP's indicate a severely altered Upoprotein structure of the sarcolemma. In addition, the necks of caveolae open and the caveolae become flattened in the plane of the membrane. With reoxygenation, many rupture. Spherical projections of cytoplasmic vesicles appear in the membrane (possibly of sarcoplasmic reticulum or lysosomal origin) and also can be seen to rupture after reoxygenation. When glucose is present in the perfusate, it affords some protec-tion against these structural defects. We propose that the fragmentation or holes in the sarco-lemma reported in severe anoxia are directly related to the structural changes reported in this study. CircRes 47:131-143, 1980