Recurrent Plasmodium falciparum malaria infections in Kenyan children diminish T-cell immunity to Epstein Barr virus lytic but not latent antigens.
Recurrent Plasmodium falciparum malaria infections in Kenyan children diminish T-cell immunity to Epstein Barr virus lytic but not latent antigens.
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DOI：
10.1371/journal.pone.0031753
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发表时间：
2012-01-01
期刊：
影响因子：
3.7
通讯作者：
Moormann, Ann M
中科院分区：
文献类型：
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作者：
Snider, Cynthia J;Cole, Stephen R;Moormann, Ann M
恶性疟原虫疟疾（Pf-malaria）和爱泼斯坦巴尔病毒（EBV）感染在有地方性伯基特淋巴瘤（eBL）风险的儿童中共存;然而，研究仅瞥见了Pf-malaria对EBV特异性免疫的累积效应。从2002年至2004年，在肯尼亚儿童（10个月至15岁）中，使用合并的EBV裂解和潜伏性CD 8 + T细胞表位肽，对IFN-γ ELISPOT应答进行了三次调查。流行率（PR）和95%置信区间（CI）估计与PF-疟疾暴露，定义在地区一级（基苏穆：全流行;南迪：低流行）和个人水平。我们观察到居住在基苏穆的5-9岁儿童与居住在南迪的5-9岁儿童相比，EBV裂解抗原IFN-γ阳性应答减少46%（PR：0.54; 95% CI：0.30-0.99）。在基苏穆的个体水平分析显示，与从未感染的儿童相比，持续感染Pf疟疾的5-9奥尔兹的EBV裂解抗原反应进一步受损。没有观察到地区或个人层面的差异之间的Pf-malaria暴露和EBV潜伏抗原IFN-γ反应。原发感染后EBV裂解抗原而非潜伏抗原IFN-γ应答的逐渐减少提示居住在疟疾完全流行区的儿童在裂解周期中免疫控制的特定丧失，进一步完善了我们对EBL病因的理解。
Plasmodium falciparum malaria (Pf-malaria) and Epstein Barr Virus (EBV) infections coexist in children at risk for endemic Burkitt's lymphoma (eBL); yet studies have only glimpsed the cumulative effect of Pf-malaria on EBV-specific immunity. Using pooled EBV lytic and latent CD8+ T-cell epitope-peptides, IFN-gamma ELISPOT responses were surveyed three times among children (10 months to 15 years) in Kenya from 2002-2004. Prevalence ratios (PR) and 95% confidence intervals (CI) were estimated in association with Pf-malaria exposure, defined at the district-level (Kisumu: holoendemic; Nandi: hypoendemic) and the individual-level. We observed a 46% decrease in positive EBV lytic antigen IFN-gamma responses among 5-9 year olds residing in Kisumu compared to Nandi (PR: 0.54; 95% CI: 0.30-0.99). Individual-level analysis in Kisumu revealed further impairment of EBV lytic antigen responses among 5-9 year olds consistently infected with Pf-malaria compared to those never infected. There were no observed district- or individual-level differences between Pf-malaria exposure and EBV latent antigen IFN-gamma response. The gradual decrease of EBV lytic antigen but not latent antigen IFN-gamma responses after primary infection suggests a specific loss in immunological control over the lytic cycle in children residing in malaria holoendemic areas, further refining our understanding of eBL etiology.