Effect of temperature and sunlight on the stability of human adenoviruses and MS2 as fecal contaminants on fresh produce surfaces
Effect of temperature and sunlight on the stability of human adenoviruses and MS2 as fecal contaminants on fresh produce surfaces
复制标题
DOI：
10.1016/j.ijfoodmicro.2013.04.007
复制
发表时间：
2013-06-17
影响因子：
5.4
通讯作者：
Girones, Rosina
中科院分区：
文献类型：
--
作者：
Carratala, Anna;Rodriguez-Manzano, Jesus;Girones, Rosina
确定附着在软质水果和沙拉蔬菜表面的食源性病毒病原体的稳定性或在感染状态下的持久性，对于支持食品安全风险评估研究至关重要。本文以生菜和草莓为代表，研究了温度和光照对传染性2型人腺病毒和MS2噬菌体在生菜和草莓表面稳定性的影响。选择人类腺病毒是因为它们作为病毒病原体和人类粪便污染的病毒指示物的双重作用。将人工污染的新鲜样品在黑暗中或在4和30摄氏度的阳光下曝晒24小时，进行稳定性分析。结果表明，温度是影响新鲜农产品表面hav稳定性的主要因素，在30摄氏度下24小时后，hav的衰减在3到4 log之间。这项工作为改善食品安全提供了有用的信息，可以考虑通过供应链传播食源性病毒。(c) 2013 Elsevier B.V.版权所有
Determining the stability, or persistence in an infectious state, of foodborne viral pathogens attached to surfaces of soft fruits and salad vegetables is essential to underpin risk assessment studies in food safety. Here, we evaluate the effect of temperature and sunlight on the stability of infectious human adenoviruses type 2 and MS2 bacteriophages on lettuce and strawberry surfaces as representative fresh products. Human adenoviruses have been selected because of their double role as viral pathogens and viral indicators of human fecal contamination. Stability assays were performed with artificially contaminated fresh samples kept in the dark or under sunlight exposure at 4 and 30 degrees C over 24 h. The results indicate that temperature is the major factor affecting HAdV stability in fresh produce surfaces, effecting decay between 3 and 4 log after 24 h at 30 degrees C. The inactivation times to achieve a reduction between 1 and 4-log are calculated for each experimental condition. This work provides useful information to be considered for improving food safety regarding the transmission of foodborne viruses through supply chains. (c) 2013 Elsevier B.V. All rights reserved.