Genome-wide identification of sweet orange (Citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.
Genome-wide identification of sweet orange (Citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.
复制标题
DOI：
10.3389/fpls.2015.00607
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Xu J;Xu H;Liu Y;Wang X;Xu Q;Deng X
在真核生物中，组蛋白乙酰化和甲基化参与调节多种发育过程和植物防御。这些组蛋白修饰事件由一系列组蛋白修饰基因家族控制。迄今为止，还没有关于柑橘属物种中组蛋白修饰相关基因的全基因组表征的研究。基于两个已测序的甜橙橙子基因组数据库，共鉴定出136个组蛋白修饰基因，包括47个组蛋白甲基转移酶基因、23个组蛋白去甲基酶基因、50个组蛋白乙酰转移酶基因和16个组蛋白去乙酰化酶基因。这些基因被分为11个基因家族。对这11个基因家族进行了染色体定位、系统发育比较、基因结构和蛋白质保守结构域组成的综合分析。为了深入了解这些基因在柑橘果实发育中的潜在作用，选择了42个在果实组织中具有高mRNA丰度的CsHMs，以进一步分析其在果实发育的6个阶段的表达谱。有趣的是，许多基因在成熟果实的果肉中高表达，其中一些基因的表达水平随着果实的发育沿着增加。此外，我们分析了所有136个CsHMs对柑橘采后过程中最具破坏性的病原菌蓝霉菌（指状青霉）感染的表达模式。结果表明，其中20个基因在果实受病原菌侵染过程中表达水平发生了强烈的变化。总之，本研究对甜橙子中的组蛋白修饰基因家族进行了全面的分析，并进一步阐明了它们在果实发育过程中的行为和对青霉感染的反应。
In eukaryotes, histone acetylation and methylation have been known to be involved in regulating diverse developmental processes and plant defense. These histone modification events are controlled by a series of histone modification gene families. To date, there is no study regarding genome-wide characterization of histone modification related genes in citrus species. Based on the two recent sequenced sweet orange genome databases, a total of 136 CsHMs (Citrus sinensis histone modification genes), including 47 CsHMTs (histone methyltransferase genes), 23 CsHDMs (histone demethylase genes), 50 CsHATs (histone acetyltransferase genes), and 16 CsHDACs (histone deacetylase genes) were identified. These genes were categorized to 11 gene families. A comprehensive analysis of these 11 gene families was performed with chromosome locations, phylogenetic comparison, gene structures, and conserved domain compositions of proteins. In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development. Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development. Furthermore, we analyzed the expression patterns of all 136 CsHMs response to the infection of blue mold (Penicillium digitatum), which is the most devastating pathogen in citrus post-harvest process. The results indicated that 20 of them showed the strong alterations of their expression levels during the fruit-pathogen infection. In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.