Appearance of alpha-smooth muscle actin in human eye lens cells of anterior capsular cataract and in cultured bovine lens-forming cells.
Appearance of alpha-smooth muscle actin in human eye lens cells of anterior capsular cataract and in cultured bovine lens-forming cells.
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α-平滑肌肌动蛋白在人眼前囊白内障晶状体细胞和培养的牛晶状体形成细胞中的出现。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Giulio Gabbiani
中科院分区：
文献类型：
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作者：
Annette Schmitt;Hans Pau;Rolf Spahr;Hans M. Piper;Omar Skalli;Giulio Gabbiani
使用光学和电子显微镜免疫定位技术以及凝胶电泳与免疫印迹相结合，我们在体内和体外检查了正常人胎儿、儿童和成人晶状体、人前囊白内障和牛晶状体细胞中细胞骨架蛋白的表达。在本报告中，我们重点观察体内正常和病理情况以及培养条件下肌动蛋白异构体的表达模式。我们注意到，发育和成熟的人类晶状体细胞以及原位牛晶状体细胞仅含有β-和γ-肌动蛋白。相比之下，α-平滑肌（α-sm）肌动蛋白是平滑肌分化的典型亚型，在不同培养时间的牛晶状体细胞中得到证实。此外，在人前囊白内障的囊下区观察到的多层细胞的特征是存在α-sm肌动蛋白。因此，肌动蛋白异构体表达的广泛变化发生在培养物中生长的晶状体细胞中，并且也可能发生在白内障发生期间。讨论了外胚层衍生的晶状体形成细胞中肌样分化标志物出现的生物学意义。
Using light and electron-microscopic immunolocalization techniques, and gel electrophoresis combined with immunoblotting, we have examined the expression of cytoskeletal proteins in normal human fetal, child and adult lenses, in human anterior capsular cataract and in bovine lens cells in vivo and in vitro. In this report, we focus our observations on the pattern of actin-isoform expression during normal and pathological situations in vivo and culture conditions. We have noted that cells of developing and mature human lenses as well as bovine lens cells in situ contain only beta- and gamma-actins. In contrast, alpha-smooth muscle (alpha-sm) actin, an isoform typical of smooth muscle differentiation, was demonstrated in bovine lens cells at different times of culture. Moreover, the multilayered cells observed in the subcapsular zone of human anterior capsular cataract were characterized by the presence of alpha-sm actin. Thus, extensive changes in actin-isoform expression take place in lens cells growing in culture and may also occur during cataractogenesis. The biological meaning of the appearance of a marker of myoid differentiation in the ectodermally derived lens-forming cells is discussed.