A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation
A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation
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DOI：
10.1523/jneurosci.21-16-06000.2001
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发表时间：
2001-08-15
影响因子：
5.3
通讯作者：
Matsui, H
中科院分区：
文献类型：
--
作者：
Matsushita, M;Tomizawa, K;Matsui, H
已经证明蛋白质和肽通过蛋白质转导结构域穿透真核细胞的质膜。我们表明，11精氨酸（11 R）的蛋白转导是一种有效的方法，提供蛋白质到脑片的神经元。在这里，我们证明PKA抑制肽，融合11 R和核定位信号，提供肽专门进入脑切片神经元的核隔室。这种抑制肽阻断cAMP反应元件结合蛋白磷酸化和持久的长时程增强（LTP）诱导，但不早期LTP。这些结果突出了蛋白质和肽转导到脑切片中的特定神经元亚细胞区室作为研究神经元可塑性的有力工具。
Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that protein transduction by 11 arginine (11R) is an efficient method of delivering proteins into the neurons of brain slices. Here, we demonstrate that PKA inhibitory peptide, fused with 11R and nuclear localization signal, delivers the peptide exclusively into the nuclear compartment of neurons in brain slices. This inhibitory peptide blocked both cAMP responsive element-binding protein phosphorylation and long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight transduction of proteins and peptides into specific neuronal subcellular compartments in brain slices as a powerful tool for studying neuronal plasticity.