Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates
Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates
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DOI：
10.4269/ajtmh.1997.57.512
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发表时间：
1997-11-01
影响因子：
3.3
通讯作者：
Nichol, ST
中科院分区：
文献类型：
--
作者：
Rodriguez, LL;Maupin, GO;Nichol, ST
1994 年至 1995 年间，在对阿拉伯联合酋长国 (UAE) 爆发的克里米亚-刚果出血热 (CCHF) 疫情进行调查期间，通过抗原捕获 ELISA 和逆转录聚合酶链式反应，对疑似 CCHF 病例的血液样本和从牲畜身上采集的蜱进行了 CCHF 病毒检测。对来自 4 个蜱虫和 5 个人类样本的部分小 (S) 片段核苷酸序列的系统发育分析表明，除了一个例外，所有人类和蜱病毒与来自巴基斯坦和马达加斯加的样本一起聚集在一个不同的谱系中。在这个谱系中，来自阿联酋患者的序列与来自三种玻璃瘤属的序列相同或密切相关。从最近从索马里进口的牲畜身上获得的蜱虫。来自阿联酋患者的另一个序列与来自尼日利亚的 CCHF 病毒关系更密切。这些数据表明，1994-1995年阿联酋的CCHF流行是多源爆发，可能与输入感染CCHF病毒的牲畜和蜱有关。
During the investigation of an outbreak of Crimean-Congo hemorrhagic fever (CCHF) in the United Arab Emirates (UAE) between 1994 and 1995, blood samples from suspected CCHF cases and ticks collected from livestock were tested for CCHF virus by antigen-capture ELISA and by a reverse transcription-polymerase chain reaction. Phylogenetic analysis of partial small (S) segment nucleotide sequences from four ticks and five human samples showed that with one exception, all the human and tick viruses clustered along with samples from Pakistan and Madagascar in one distinct lineage. Within this lineage, sequences from the UAE patients were identical or closely related to those from three Hyalomma spp. ticks obtained from livestock recently imported from Somalia. Another sequence from a UAE patient was more closely related to a CCHF virus from Nigeria. These data indicate that the 1994-1995 CCHF epidemic in the UAE was a multisource outbreak possibly associated with importation of CCHF virus-infected livestock and ticks.