PCR detection of white spot syndrome virus (WSSV) from farmed Pacific white shrimp (Litopenaeus vannamei) in selected sites of the Philippines.
PCR detection of white spot syndrome virus (WSSV) from farmed Pacific white shrimp (Litopenaeus vannamei) in selected sites of the Philippines.
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PCR 检测菲律宾选定地点养殖的太平洋白虾（Litopenaeus vannamei）中的白斑综合症病毒（WSSV）。
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
M. B. Maningas
中科院分区：
文献类型：
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作者：
B. Maralit;C. Caipang;Mudjekeewis D. Santos;M. B. Maningas
对虾白斑综合症病毒(WSSV)在对虾养殖中造成的巨大损失归因于养殖场不佳的筛选程序和缺乏足够的无特定病原体的育苗库。因此，及早发现病毒被认为是养虾户的最佳选择。因此，这项研究评估了菲律宾的病毒发病率，并将菲律宾的WSSV分离株与GenBank中的其他分离株进行了部分测序和特征比较。所研制的聚合酶链式反应引物能从0.4pg的对虾DNA提取液中检测到目的基因。聚合酶链式反应检测显示，来自赞巴莱的市场样品中有6.67%(1/15)感染了WSSV。从桑托斯市当地一家商店和一家公共市场采集的对虾样本中，WSSV阳性样本分别为46.67%(7/15)和20%(3/15)。然而，来自Capiz和Batangas的虾来源显示WSV检测为阴性。来自采样点的感染样本数量没有明显差异。联合检测显示，菲律宾的感染率较低，为14.67%。这项研究已经对菲律宾分离株进行了部分测序和特征分析。在抽样期内，桑托斯市的大多数虾经聚合酶链式反应检测呈WSSV阳性。
Great losses caused by white spot syndrome virus (WSSV) in shrimp culture have been attributed to poor screening procedures in farms and the lack of sufficient access to specific pathogen free brood stock. Thus, early detection of the virus is considered the best option for shrimp farmers. The study, thus, assessed viral incidence in the Philippines and partially sequenced and characterized the Philippine WSSV isolate with regards to other isolates in GenBank. Developed primers for PCR can detect target genes from 0.4 pg of DNA extract from shrimp samples. PCR detection revealed that 6.67 % (1/15) of market samples from Zambales are infected with WSSV. Shrimp samples from a local shop and a public market in General Santos City showed 46.67% (7/15) and 20% (3/15) WSSV-positive samples respectively. Shrimp sources from Capiz and Batangas, however, showed negative detection for WSV. No significant difference in the number of infected samples from the sampling sites was found. Combined detections reveal that the Philippines have a low infection rate of 14.67%. The study has partially sequenced and characterized Philippine isolate. During the sampling period, most shrimps in General Santos City were WSSV-positive by PCR detection.