THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE MITOCHONDRIAL-DNA OF THE FIN WHALE, BALAENOPTERA-PHYSALUS
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE MITOCHONDRIAL-DNA OF THE FIN WHALE, BALAENOPTERA-PHYSALUS
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DOI：
10.1007/bf02102808
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发表时间：
1991-12-01
影响因子：
3.9
通讯作者：
WIDEGREN, B
中科院分区：
文献类型：
--
作者：
ARNASON, U;GULLBERG, A;WIDEGREN, B
测定了长须鲸线粒体DNA的组成。该分子长度为16,398 bp，其结构与其他哺乳动物的结构一致。长须鲸和牛的mtDNA之间的一般相似性大于长须鲸和其他物种（人类，小鼠，大鼠）之间的相似性，其中整个分子的组成已经被描述。长须鲸线粒体DNA的D环区域与海豚DNA的D环有81%的相同性，D环的中心部分与牛的D环相似。通过比较长须鲸、牛和人类，评估了12 S和16 S rRNA基因中颠换和缺口的积累。人与鲸、人与牛之间的序列差异在同一水平上，表明偶蹄类和鲸类mtDNA rRNA基因的进化速率大致相同。在12 S rRNA基因中，每百万年0.05%的积累率将鲸类和偶蹄类的分离定位在大约5500万年前。人类和鲸鱼或牛的相应数字约为8000万年。在16 S rRNA基因中，每百万年0.68%的颠换和缺口积累率产生了一致的数字。通过对长须鲸的细胞色素B基因和文献中的细胞色素B序列（包括海豚（Stenella）序列）进行比较，确定鲸目动物为单系动物，偶蹄动物为它们最近的亲属。长须鲸和斯氏鲸的细胞色素B序列之间的比较表明，密码子位置1或2的差异经常与另一个密码子位置的变化有关。
The composition of the mitochondrial DNA (mtDNA) of the fin whale, Balaenoptera physalus, was determined. The length of the molecule is 16,398 bp, and its organization conforms with that of other mammals. The general similarity between the mtDNA of the fin whale and the cow is greater than the similarity between the fin whale and other species (human, mouse, rat) in which the composition of the entire molecule has been described. The D-loop region of the mtDNA of the fin whale is 81 % identical to the D-loop of dolphin DNA, and the central portion of the D-loop is similar to the bovine D-loop. The accumulation of transversions and gaps in the 12S and 16S rRNA genes was assessed by comparing the fin whale, cow, and human. The sequence difference between human and the whale and human and the cow was at the same level, indicating that the rate of evolution of the mtDNA rRNA genes is about the same in artiodactyls and cetaceans. In the 12S rRNA gene an accumulation rate of 0.05% per million years places the separation of cetaceans and artiodactyls at about 55 million years ago. The corresponding figure for human and either the whale or the cow is about 80 million years. In the 16S rRNA gene a 0.68% accumulation rate of transversions and gaps per million years yields concurring figures. A comparison between the cytochrome b gene of the fin whale and cytochrome b sequences in the literature, including dolphin (Stenella) sequences, identified the cetaceans as monophyletic and the artiodactyls as their closest relatives. The comparison between the cytochrome b sequences of the fin whale and Stenella showed that differences in codon positions one or two were frequently associated with a change in another codon position.