The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep
The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep
复制标题
CRISPR/Cas9 诱导 MSTN 基因组大片段缺失和绵羊表型变化
DOI：
10.1016/s2095-3119(19)62853-4
复制
发表时间：
2020-04-01
影响因子：
4.8
通讯作者：
Chen Yu-lin
中科院分区：
文献类型：
--
作者：
Ding Yi;Zhou Shi-wei;Chen Yu-lin
CRISPR/Cas9系统已被广泛用于工程化遗传基因座，以在动物模型中产生敲除、插入和点突变。然而，在动物中报道的许多突变是小的插入或缺失。这项研究使用CRISPR/Cas9系统通过绵羊中的三种指导RNA诱导MIBI中的大DNA片段缺失。这通过将Cas9 mRNA和sgRNA注射到单细胞阶段的胚胎中，成功实现了绵羊MIBR基因的精确基因编辑。在10只编辑的动物中，3只动物（30%）表现出大的基因组片段缺失（类似于5 kb）。此外，这3只动物的体重差异显著（P-0
The CRISPR/Cas9 system has been extensively used to engineer genetic loci for the generation of knockouts, insertions, and point mutations in animal models. However, many mutations that have been reported in animals are small insertions or deletions. This study used the CRISPR/Cas9 system to induce large DNA fragment deletions in MSTN via three guide RNAs in sheep. This successfully achieved the precise gene editing of the ovine MSTN gene by injecting both Cas9 mRNA and sgRNAs into embryos at the one-cell stage. Of 10 edited animals, 3 animals (30%) exhibited large genomic fragment deletions (similar to 5 kb). Furthermore, the body weights of these 3 animals were significantly different (P-0