Effects of Dietary Yogurt on the Healthy Human Gastrointestinal (GI) Microbiome.
Effects of Dietary Yogurt on the Healthy Human Gastrointestinal (GI) Microbiome.
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DOI：
10.3390/microorganisms5010006
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发表时间：
2017-02-15
期刊：
影响因子：
4.5
通讯作者：
Johnston CG
中科院分区：
文献类型：
--
作者：
Lisko DJ;Johnston GP;Johnston CG
胃肠道（GI）执行调节宿主和微生物群之间关系的关键功能。研究表明，益生菌摄入在调节免疫反应和人体代谢过程中具有许多益处。然而，不利的注意力已经支付的时间变化的微生物组成和多样性的胃肠道。这项研究旨在研究酸奶消费对胃肠道微生物群落组成，结构和多样性的影响，在短期内（42天）。我们使用了一种结合经典指纹技术（T-RFLP）、桑格分析和Illumina MiSeq 16 S rRNA基因扩增子测序的多方法来阐明每天食用高剂量酸奶的健康成人中的细菌群落以及乳酸杆菌和双歧杆菌细菌种群。结果表明，总体GI微生物群落和多样性是方法依赖性的，但我们发现在整个研究期间食用高剂量酸奶的健康受试者中细菌组成和结构的个体特定变化。
The gastrointestinal (GI) tract performs key functions that regulate the relationship between the host and the microbiota. Research has shown numerous benefits of probiotic intake in the modulation of immune responses and human metabolic processes. However, unfavorable attention has been paid to temporal changes of the microbial composition and diversity of the GI tract. This study aimed to investigate the effects of yogurt consumption on the GI microbiome bacteria community composition, structure and diversity during and after a short-term period (42 days). We used a multi-approach combining classical fingerprinting techniques (T-RFLPs), Sanger analyses and Illumina MiSeq 16S rRNA gene amplicon sequencing to elucidate bacterial communities and Lactobacilli and Bifidobacteria populations within healthy adults that consume high doses of yogurt daily. Results indicated that overall GI microbial community and diversity was method-dependent, yet we found individual specific changes in bacterial composition and structure in healthy subjects that consumed high doses of yogurt throughout the study.