Plasmid-mediated fitness advantage of Acinetobacter baylyi in sulfadiazine-polluted soil.
Plasmid-mediated fitness advantage of Acinetobacter baylyi in sulfadiazine-polluted soil.
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磺胺嘧啶污染土壤中贝氏不动杆菌介导的适应性优势
DOI：
10.1111/1574-6968.12284
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发表时间：
2013
影响因子：
2.1
通讯作者：
Smalla K
中科院分区：
文献类型：
--
作者：
Jechalke S;Kopmann C;Richter M;Moenickes S;Heuer H;Smalla K
对磺胺具有抗性的低gc型质粒经常从粪肥和肥料土壤中分离出来。然而，关于土壤中质粒携带种群的动态及其对磺胺存在的反应的知识很少。本研究研究了在施用或不施用磺胺类抗生素磺胺嘧啶（SDZ）的肥料后，具有磺胺抗性的质粒pHHV216对baylyiBD413在土壤中的适合度的影响。a的持久性。在土壤微观环境中，对baylyiBD413 pHHV216与其无质粒变体的竞争进行了观察。CFU计数下降。在32天的试验期内，baylyiBD413在施用肥料的土壤中增加了约0.5 log单位。在不添加SDZ时，携带质粒的群体比例从50%下降到< 40%，而添加SDZ后，携带BD413的质粒比例从50%上升到约65%。这些数据表明，通过粪便导入土壤的SDZ具有生物可达性，这为携带质粒的BD413在土壤中的群体提供了适应性优势，而在没有SDZ选择压力的情况下，质粒则带来了适应性劣势。该方法可作为抗生素在土壤中生物利用度评价的工具。
LowGC-type plasmids conferring resistance to sulfonamides have been frequently isolated from manure and manured soil. However, knowledge on the dynamics of plasmid-carrying populations in soil and their response to the presence of sulfonamides is scarce. Here, we investigated effects of the sulfonamide resistance conferring plasmid pHHV216 on the fitness ofAcinetobacter baylyiBD413 in soil after application of manure with or without the sulfonamide antibiotic sulfadiazine (SDZ). The persistence ofA. baylyiBD413 pHHV216 in competition to its plasmid-free variant was followed in soil microcosms. CFU counts showed a decrease inA. baylyiBD413 in manured soils over the experimental period of 32 days by about 0.5 log units. The proportion of the plasmid-carrying populations decreased from 50 to < 40% in the absence of SDZ, while the proportion of plasmid-carrying BD413 increased from 50 to about 65% with SDZ added. The data suggest that SDZ introduced via manure into soil was bioaccessible, providing a fitness advantage for the plasmid-carrying population of BD413 in soil, while the plasmid conferred a fitness disadvantage when selective pressure by SDZ was absent. In future, this method may be used as a tool for the assessment of bioavailability of antibiotics in soil.
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影响因子：
4.2
作者：
Kopmann, Christoph;Jechalke, Sven;Smalla, Kornelia
通讯作者：
Smalla, Kornelia
影响因子：
4.4
作者：
Jechalke, Sven;Kopmann, Christoph;Smalla, Kornelia
通讯作者：
Smalla, Kornelia
影响因子：
9.7
作者：
Heuer, Holger;Focks, Andreas;Spiteller, Michael
通讯作者：
Spiteller, Michael
影响因子：
11.4
作者：
Foerster, M.;Laabs, V.;Amelung, W.
通讯作者：
Amelung, W.
影响因子：
11.4
作者：
Ingrid Rosendahl;J. Siemens;J. Groeneweg;Elisabeth Linzbach;V. Laabs;C. Herrmann;H. Vereecken;W. Amelung
通讯作者：
Ingrid Rosendahl;J. Siemens;J. Groeneweg;Elisabeth Linzbach;V. Laabs;C. Herrmann;H. Vereecken;W. Amelung