Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells
Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells
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DOI：
10.1046/j.1471-4159.2003.01895.x
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发表时间：
2003-08-01
影响因子：
4.7
通讯作者：
Gray, WP
中科院分区：
文献类型：
--
作者：
Howell, OW;Scharfman, HE;Gray, WP
新神经元终生在成年的海马体中产生，是某些类型的海马体学习所必需的。然而，对海马神经发生的控制知之甚少。我们使用出生后早期大鼠和神经肽Y Y1受体敲除小鼠的原代海马区培养，以及选择性神经肽Y受体拮抗剂和激动剂，证明神经肽Y对巢蛋白阳性的、球状形成的海马前体细胞和β微管蛋白阳性的神经母细胞具有增殖作用，神经肽Y的神经增殖作用是通过其Y1受体介导的。免疫组织化学证实培养的Nestin阳性细胞和β-微管蛋白阳性细胞均表达Y1受体，短脉冲5-溴-2-脱氧尿嘧啶核苷研究表明神经肽Y对这两种细胞均有增殖作用。这些研究表明，神经肽Y促进了海马神经母细胞和前体细胞的增殖，因此，神经肽Y释放的神经元间质可能对海马的学习和记忆起调节作用。
New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post-natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin-positive, sphere-forming hippocampal precursor cells and beta-tubulin-positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin-positive cells and beta-tubulin-positive cells in culture and short pulse 5-bromo-2-deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y-releasing interneurones.