23Na NMR study of Fibrobacter succinogenes S85: comparison of three chemical shift reagents and calculation of sodium concentration using ionophores.
23Na NMR study of Fibrobacter succinogenes S85: comparison of three chemical shift reagents and calculation of sodium concentration using ionophores.
复制标题
Fibrobacter succinogenes S85 的 23Na NMR 研究：三种化学位移试剂的比较以及使用离子载体计算钠浓度。
DOI：
10.1006/abio.2002.5685
复制
发表时间：
2002
影响因子：
2.9
通讯作者：
E. Forano
中科院分区：
文献类型：
--
作者：
A. Delort;G. Gaudet;E. Forano
为了用（23）Na NMR光谱法测定产琥珀酸纤维杆菌S85静息细胞内钠离子浓度，对两种方法进行了研究。首先，测试了三种不同的位移试剂（Dy（PPP（i））（7-）（2）、Tm（DOTP）（5-）和Dy（TTHA）（3-））分离内部和外部（23）Na NMR共振的能力。对F.通过体内（13）C NMR实验评价产琥珀酸菌细胞。发现Tm（DOTP）（5-）是最有效的转换试剂，同时无毒。第二，发展了一种新的计算细胞内钠离子浓度的方法。通过使用离子载体合成琥珀酸酯。这种方法避免了细胞内容积测量和钠可见度测定的问题。
In order to measure intracellular sodium concentrations in resting cells of Fibrobacter succinogenes S85 by (23)Na NMR spectrometry, two methodological aspects were studied. First, three different shift reagents (Dy(PPP(i))(7-)(2), Tm(DOTP)(5-), and Dy(TTHA)(3-)) were tested for their ability to separate internal and external (23)Na NMR resonances. Their toxicity toward F. succinogenes cells was evaluated by in vivo(13)C NMR experiments. Tm(DOTP)(5-) was found to be the most efficient shift reagent while being nontoxic. Second, a new methodology was developed to calculate intracellular sodium concentration in F. succinogenes by using ionophores. This approach avoided the problem of intracellular volume measurement and that of sodium visibility determination.
通过高分辨率金属阳离子 NMR 测量组织中的金属阳离子区室化。
DOI：
10.1146/annurev.bb.16.060187.002111
发表时间：
1987
期刊：
Annual review of biophysics and biophysical chemistry
影响因子：
--
作者：
SpringerJr,CS
通讯作者：
SpringerJr,CS