Regionally variant collagen alignment correlates with viscoelastic properties of the disc of the human temporomandibular joint
Regionally variant collagen alignment correlates with viscoelastic properties of the disc of the human temporomandibular joint
复制标题
DOI：
10.1016/j.archoralbio.2017.11.002
复制
发表时间：
2018-02-01
影响因子：
3
通讯作者：
Lee, Chang H.
中科院分区：
文献类型：
--
作者：
Gutman, Shawn;Kim, Daniel;Lee, Chang H.
目的：为了确定区域变化的质量，胶原蛋白排列在人类颞下颌关节盘和其统计相关性与粘弹性properties.Design：胶原蛋白排列的质量的定量分析，水平部分的人类颞下颌关节盘Pricrosirius红染色成像圆偏振显微镜下。使用成熟的角梯度自动图像处理分析每个区域中胶原纤维的平均角和角偏差。在拉伸和压缩的应力松弛试验下，测量每个圆盘区域的瞬时模量和松弛模量。然后对角度偏差与模量进行了斯皮尔曼相关分析。为了了解糖胺聚糖对相关性的影响，用软骨素酶ABC（C-ABC）处理TMJ盘样本。结果：我们的图像处理分析显示胶原排列的区域变异方向，与以前的研究结果一致。有趣的是，胶原蛋白排列的质量，不仅是方向，在区域之间是显著不同的。前、中间区纤维排列的角度偏差明显小于后区。内侧区和外侧区的角度偏差明显大于其他区域。区域变化的角度偏差值表现出统计学显着的相关性与拉伸瞬时模量和松弛模量，部分依赖于C-ABC treatment.Conclusion：我们的研究结果表明，区域变化程度的胶原纤维排列可能是由于颞下颌关节盘的异质粘弹性，可能有显着的影响，在开发再生治疗颞下颌关节盘。
Objective: To determine the regionally variant quality of collagen alignment in human TMJ discs and its statistical correlation with viscoelastic properties.Design: For quantitative analysis of the quality of collagen alignment, horizontal sections of human TMJ discs with Pricrosirius Red staining were imaged under circularly polarized microscopy. Mean angle and angular deviation of collagen fibers in each region were analyzed using a well-established automated image-processing for angular gradient. Instantaneous and relaxation moduli of each disc region were measured under stress relaxation test both in tensile and compression. Then Spearman correlation analysis was performed between the angular deviation and the moduli. To understand the effect of glycosaminoglycans on the correlation, TMJ disc samples were treated by chondroitinase ABC (C-ABC).Results: Our imaging processing analysis showed the region-variant direction of collagen alignment, consistently with previous findings. Interestingly, the quality of collagen alignment, not only the directions, was significantly different in between the regions. The angular deviation of fiber alignment in the anterior and intermediate regions were significantly smaller than the posterior region. Medial and lateral regions showed significantly bigger angular deviation than all the other regions. The regionally variant angular deviation values showed statistically significant correlation with the tensile instantaneous modulus and the relaxation modulus, partially dependent on C-ABC treatment.Conclusion: Our findings suggest the region-variant degree of collagen fiber alignment is likely attributed to the heterogeneous viscoelastic properties of TMJ disc that may have significant implications in development of regenerative therapy for TMJ disc.