Induction of murine T-helper-cell responses to the filarial nematode Brugia malayi.
Induction of murine T-helper-cell responses to the filarial nematode Brugia malayi.
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诱导小鼠 T 辅助细胞对丝虫线虫马来丝虫的反应。
DOI：
10.1128/iai.61.3.1105-1112.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Kazura,JW
中科院分区：
文献类型：
--
作者：
Pearlman,E;HazlettJr,FE;Boom,WH;Kazura,JW
本研究的目的是研究小鼠t辅助细胞（Th）细胞因子对人马来布鲁氏丝虫病的反应。在BALB/c小鼠腹腔注射活微丝虫病后的头14天，丝虫病抗原驱动的脾淋巴样细胞产生γ干扰素（ifn - γ）和少量或不产生白细胞介素-5 （IL-5）。在此之后，IL-5的产生增加（每5 × 10(6)个细胞10至12 ng），同时ifn - γ的产生明显减少。用可溶性微丝抗原进行单次皮下免疫也能诱导ifn - γ，但没有IL-5反应。而免疫三次引起主要的Th2样反应，其特征是CD4+淋巴结淋巴细胞产生IL-4和IL-5，血清免疫球蛋白e增加10倍。IL-10在建立寄生虫特异性Th1和Th2反应之间的平衡中的重要性，可以通过中和该细胞因子的单克隆抗体来增加长期暴露于活微丝虫的小鼠脾和淋巴结细胞产生ifn - γ的能力来证明或者用可溶性丝虫病抗原多次免疫。
The purpose of this study was to examine the murine T-helper-cell (Th) cytokine response to the human filarial parasite Brugia malayi. In the first 14 days following intraperitoneal inoculation of live microfilariae into BALB/c mice, filarial antigen-driven splenic lymphoid cells produced gamma interferon (IFN-gamma) and little or no interleukin-5 (IL-5). After this time, IL-5 production increased (to 10 to 12 ng per 5 x 10(6) cells) coincident with a marked diminution in IFN-gamma generation. A single subcutaneous immunization with soluble microfilarial antigens also induced an IFN-gamma but no IL-5 response, whereas immunization three times elicited a predominant Th2-like reaction characterized by IL-4 and IL-5 production by CD4+ lymph node lymphocytes and a 10-fold increase in serum immunoglobulin E. The importance of IL-10 in establishing the balance between parasite-specific Th1 and Th2 responses was demonstrated by the ability of neutralizing monoclonal antibody to this cytokine to increase IFN-gamma production by splenic and lymph node cells from mice chronically exposed to live microfilariae or immunized multiple times with soluble filarial antigens.