Comparative mitochondrial genome analysis of Dendrolimus houi (Lepidoptera: Lasiocampidae) and phylogenetic relationship among Lasiocampidae species
Comparative mitochondrial genome analysis of Dendrolimus houi (Lepidoptera: Lasiocampidae) and phylogenetic relationship among Lasiocampidae species
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胡伊松毛虫（鳞翅目：毛毛虫科）线粒体基因组比较分析及毛毛虫科物种之间的系统发育关系
DOI：
10.1371/journal.pone.0232527
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发表时间：
2020-05-14
期刊：
影响因子：
3.7
通讯作者：
Liang，Guanghong
中科院分区：
文献类型：
--
作者：
Han，Xiaohong;He，Huan;Liang，Guanghong
云南松毛虫（Dendrolimus houi）是危害裸子植物最常见的毛虫之一，广泛分布于东南亚一些国家，在不同的森林生境中单独或与几种同类和一些松毛虫科（Lasiocampidae）物种共存。然而，自然杂交偶尔发生在一些密切相关的物种在同一生境中，和主机的偏好，极端的气候压力，地理隔离可能导致他们的不确定的分类共识。线粒体DNA（mtDNA）序列分析表明，D.利用高通量技术提取houi基因并测序，比较分析其有丝分裂基因组的组成和特征，基于13个蛋白质编码基因数据集，采用最大似然法（ML）和贝叶斯法（BI）构建系统发育关系，它们被合并并提供下载，这些数据被合并并提供下载，在全球Lasiocampidae物种数据中。D. houi基因全长15，373 bp，包含37个基因，其中PCG基因13个，tRNA基因（tRNAs）22个，rRNA基因（rRNAs）2个。基因的位置和序列与已知的大多数Lasiocampidae物种一致。核苷酸组成是高度A+T偏倚的，占整个有丝分裂基因组的~80%。PCG的起始密码子除COI以CGA结尾外，其余均为典型的ATN起始密码子，终止密码子以标准的TAA或TAG结尾，COI、COII、ND 4以不完全T结尾。只有tRNASer（AGN）缺少DHU臂，而其余的形成典型的“三叶草形”二级结构。对于Lasiocampidae物种，其完整的线粒体基因组长度为15，281至15，570 bp，并且所有第一个基因都以相同的方向从trnM开始。碱基组成偏向于A和T。最后，两种方法（ML和BI）分别揭示了D. spp.如（D. punctatus + D. tabulaeformis）+ D. Escherichia）+ D. superans）+（D. kikuchii + D. houi），但在D.云南居群的菊池虫也被包括在内，表明不同地理居群的昆虫已经发生了分化。松毛虫科的系统发育关系为（松毛虫）+ Kunugia）+ Euthrix）+ Trabala）。这为Lasiocampidae的进化和分类提供了更好的理论依据，为未来的研究方向提供了依据。
Dendrolimus houi is one of the most common caterpillars infesting Gymnosperm trees, and widely distributed in several countries in Southeast Asia, and exists soley or coexists with several congeners and some Lasiocampidae species in various forest habitats. However, natural hybrids occasionally occur among some closely related species in the same habitat, and host preference, extreme climate stress, and geographic isolation probably lead to their uncertain taxonomic consensus. The mitochondrial DNA (mtDNA) of D. houi was extracted and sequenced by using high-throughput technology, and the mitogenome composition and characteristics were compared and analyzed of these species, then the phylogenetic relationship was constructed using the maximum likelihood method (ML) and the Bayesian method (BI) based on their 13 protein-coding genes (PCGs) dataset, which were combined and made available to download which were combined and made available to download among global Lasiocampidae species data. Mitogenome of D. houi was 15,373 bp in length, with 37 genes, including 13 PCGs, 22 tRNA genes (tRNAs) and 2 rRNA genes (rRNAs). The positions and sequences of genes were consistent with those of most known Lasiocampidae species. The nucleotide composition was highly A+T biased, accounting for ~80% of the whole mitogenome. All start codons of PCGs belonged to typical start codons ATN except for COI which used CGA, and most stop codons ended with standard TAA or TAG, while COI, COII, ND4 ended with incomplete T. Only tRNASer (AGN) lacked DHU arm, while the remainder formed a typical “clover-shaped” secondary structure. For Lasiocampidae species, their complete mitochondrial genomes ranged from 15,281 to 15,570 bp in length, and all first genes started from trnM in the same direction. And base composition was biased toward A and T. Finally, both two methods (ML and BI) separately revealed that the same phylogenetic relationship of D. spp. as ((((D. punctatus + D. tabulaeformis) + D. spectabilis) + D. superans) + (D. kikuchii of Hunan population + D. houi) as in previous research, but results were different in that D. kikuchii from a Yunnan population was included, indicating that different geographical populations of insects have differentiated. And the phylogenetic relationship among Lasiocampidae species was ((((Dendrolimus) + Kunugia) + Euthrix) + Trabala). This provides a better theoretical basis for Lasiocampidae evolution and classification for future research directions.