Shotgun proteomic analysis of nanoparticle-synthesizing Desulfovibrio alaskensis in response to platinum and palladium.
Shotgun proteomic analysis of nanoparticle-synthesizing Desulfovibrio alaskensis in response to platinum and palladium.
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DOI：
10.1099/mic.0.000840
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发表时间：
2019-12-01
期刊：
影响因子：
--
通讯作者：
Horsfall, Louise E
中科院分区：
文献类型：
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作者：
Capeness, Michael J;Imrie, Lisa;Horsfall, Louise E
铂和钯是备受追捧的金属，在丰富性和可用性方面具有至关重要的全球重要性。在纳米尺度上，这些金属由于其在工业应用中的催化能力而具有更高的价值。阿拉斯加脱硫弧菌能够捕获这两种金属的离子形式，将其还原并合成元素纳米颗粒。尽管具有这种能力，但人们对这些纳米颗粒形成所涉及的生物途径知之甚少。阿拉斯加石竹对铂和钯的反应的蛋白质组学分析强调了那些参与还原途径和更广泛的应激反应系统的蛋白质。在两种处理中都发现了一组由 13 种蛋白质组成的核心蛋白质，它们由参与金属转运和还原的蛋白质组成。还有七种特定于铂或钯的蛋白质。这些铂特异性基因之一——NiFe氢化酶小亚基（Dde_2137）的过度表达导致了更大纳米颗粒的形成。这项研究提高了我们对脱硫弧菌金属抗性机制所涉及途径的理解，并为我们如何定制细菌以生产纳米颗粒、增强其作为生物修复工具和从环境中捕获污染物金属的方法的应用提供了信息。
Platinum and palladium are much sought-after metals of critical global importance in terms of abundance and availability. At the nano-scale these metals are of even higher value due to their catalytic abilities for industrial applications. Desulfovibrio alaskensis is able to capture ionic forms of both of these metals, reduce them and synthesize elemental nanoparticles. Despite this ability, very little is known about the biological pathways involved in the formation of these nanoparticles. Proteomic analysis of D. alaskensis in response to platinum and palladium has highlighted those proteins involved in both the reductive pathways and the wider stress-response system. A core set of 13 proteins was found in both treatments and consisted of proteins involved in metal transport and reduction. There were also seven proteins that were specific to either platinum or palladium. Overexpression of one of these platinum-specific genes, a NiFe hydrogenase small subunit (Dde_2137), resulted in the formation of larger nanoparticles. This study improves our understanding of the pathways involved in the metal resistance mechanism of Desulfovibrio and is informative regarding how we can tailor the bacterium for nanoparticle production, enhancing its application as a bioremediation tool and as a way to capture contaminant metals from the environment.