Preclinical applications of high-definition manometry system to investigate pelvic floor muscle contribution to continence mechanisms in a rabbit model.
Preclinical applications of high-definition manometry system to investigate pelvic floor muscle contribution to continence mechanisms in a rabbit model.
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高清测压系统的临床前应用研究盆底肌肉对兔子模型失禁机制的贡献。
DOI：
10.1152/ajpgi.00295.2021
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Rajasekaran,MahadevanRaj
中科院分区：
文献类型：
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作者：
Sorkhi,Samuel;Seo,Youngjin;Bhargava,Valmik;Rajasekaran,MahadevanRaj
肛门外括约肌(EAS)、外道括约肌和耻骨直肠肌(PRM)在肛门和尿路闭合压力的形成中起着重要作用。在本研究中，我们使用兔模型和高清晰度测压(HDM)系统，单独和结合括约肌关闭功能来定义这些肌肉的贡献。共有12只雌性兔子被麻醉，并准备使用HDM系统测量肛管、尿路和阴道压力。记录静息和电刺激EAS和PRM时的压力。对几只兔(n=6)进行EAS损伤，观察EAS损伤对闭合压力分布的影响。EAS和PRM在静息和电刺激时记录的肛管、尿路和阴道压力显示出不同的压力曲线。刺激EAS可引起肛管压力升高，而刺激PRM可使三个肛门的肛管压力均升高。损伤后电刺激EAS可使肛管压力降低约19%。同时电刺激EAS和PRM时，与EAS或PRM单独刺激后记录的压力相比，单个肛管压力的增加并不明显。我们的数据证实，HDM是一种可行的系统，可以测量三个开口内的动态压力变化，并在临床前研究中定义每个肌肉在这些开口内闭合压力发展中的作用。NEW&NOTEWORTHY我们预计，使用这种新的HDM技术，这些开口内的生理变化可能会使用从96个传感器产生的大量数据来重新定义。与传统方法相比，HDM的优点是响应速度更快，以及利用96个圆周传感器同时测量三个孔口的压力。我们的发现表明，这项技术可能被用来更好地确定尿漏点压力。
External anal sphincter (EAS), external urethral sphincters, and puborectalis muscle (PRM) have important roles in the genesis of anal and urethral closure pressures. In the present study, we defined the contribution of these muscles alone and in combination with the sphincter closure function using a rabbit model and a high-definition manometry (HDM) system. A total of 12 female rabbits were anesthetized and prepared to measure anal, urethral, and vaginal canal pressures using a HDM system. Pressure was recorded at rest and during electrical stimulation of the EAS and PRM. A few rabbits (n= 6) were subjected to EAS injury and the impact of EAS injury on the closure pressure profile was also evaluated. Anal, urethral, and vaginal canal pressures recorded at rest and during electrical stimulation of EAS and PRM demonstrated distinct pressure profiles. EAS stimulation induced anal canal pressure increase, whereas PRM stimulation increased the pressures in all the three orifices. Electrical stimulation of EAS after injury resulted in about 19% decrease in anal canal pressure. Simultaneous electrical stimulation of EAS and PRM resulted in an insignificant increase of individual anal canal pressures when compared with pressures recorded after EAS or PRM stimulations alone. Our data confirm that HDM is a viable system to measure dynamic pressure changes within the three orifices and to define the role of each muscle in the development of closure pressures within these orifices in preclinical studies.NEW & NOTEWORTHYWe anticipate that with this new HDM technology, physiological changes within these orifices may be redefined using the extensive data that are generated from 96 sensors. When compared with conventional methods, HDM offers the advantages of an increased response rate, as well as the utilization of 96 circumferential sensors to simultaneously measure pressure along the three orifices. Our findings suggest a potential use of this technology to better define urinary leak point pressure.