RELATIVE EFFECTS OF PREGNANCY, ESTRADIOL, AND PROGESTERONE ON PLASMA INSULIN AND PANCREATIC ISLET INSULIN SECRETION
RELATIVE EFFECTS OF PREGNANCY, ESTRADIOL, AND PROGESTERONE ON PLASMA INSULIN AND PANCREATIC ISLET INSULIN SECRETION
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DOI：
10.1172/jci106593
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发表时间：
1971-01-01
影响因子：
15.9
通讯作者：
KALKHOFF, RK
中科院分区：
文献类型：
--
作者：
COSTRINI, NV;KALKHOFF, RK
本研究评估了雌激素和孕激素对妊娠期间高胰岛素血症和胰岛胰岛素分泌增加的影响。各组雌性大鼠皮下注射不同每日剂量的苯甲酸雌二醇或油中黄体酮，持续 21 天。第21天，通过胶原酶法分离胰岛。 10 个胰岛在含有葡萄糖的缓冲培养基中孵育 90 分钟后，测量总胰岛素分泌。较高生理剂量的雌二醇或黄体酮，单独或组合，显着增加了胰岛分泌，高于未治疗的对照大鼠的值，并且与足月、3周怀孕大鼠的增强的胰岛反应相当。在这些情况下，从类固醇处理的怀孕大鼠中获得的胰岛的直径和蛋白质含量超过了对照测量值。然而，对照胰岛与 1 或 10 μg/ml 任一类固醇预孵育 2 小时不会影响随后的葡萄糖刺激的胰岛素输出。 在相关研究中，在 30 分钟静脉内葡萄糖耐量测试期间，血浆胰岛素反应显着高于足月妊娠大鼠和接受相当剂量类固醇 3 周的动物的对照反应。与妊娠或黄体酮治疗不同，单独施用雌二醇或与黄体酮一起施用显着降低了攻击后血浆葡萄糖浓度。这些结果表明，雌二醇和黄体酮有助于增强妊娠期间胰岛胰岛素分泌和血浆胰岛素对葡萄糖施用的反应。这种变化不是急剧产生的，但可能与长期激素施用后胰岛肥大有关。尽管数据没有区分这些性类固醇的直接和间接β细胞营养作用，但雌二醇和黄体酮的代谢作用可能不同，因为雌激素治疗会降低诱导高胰岛素血症后的血浆葡萄糖曲线。
Influences of estrogen and progesterone on the development of hyperinsulinemia and augmented pancreatic islet insulin secretion during pregnancy were assessed in this study. Groups of female rats were injected subcutaneously for 21 days with varying daily dosages of estradiol benzoate or progesterone in oil. On day 21, pancreatic islets were isolated by a collagenase method. Total insulin secretion was measured after 90-min incubations of 10 islets in buffered medium containing glucose. Higher physiologic dosages of estradiol or progesterone, singly or in combination, significantly increased islet secretion above values of untreated control rats and were comparable to augmented islet responses of term, 3-wk pregnant rats. Diameter and protein content of islets obtained from steroid-treated and pregnant rats exceeded control measurements in these instances. However, 2-hr preincubations of control islets with 1 or 10 μg/ml of either steroid did not influence subsequent glucose-stimulated insulin output.In related studies, plasma insulin responses during 30 min intravenous glucose tolerance tests were significantly above control responses in term-pregnant rats and animals receiving comparable dosages of steroids for 3 wk. Unlike pregnancy or progesterone treatment, estradiol administration alone or with progesterone significantly lowered postchallenge plasma glucose concentrations.These results indicate that estradiol and progesterone contribute to enhanced islet insulin secretion and plasma insulin responses to glucose administration during pregnancy. This change is not acutely produced but can be related to hypertrophy of islets following chronic hormonal administration. Although the data do not distinguish between direct and indirect beta-cytotrophic effects of these sex steroids, metabolic actions of estradiol and progesterone may differ, since estrogen treatment lowers plasma glucose curves following the induction of hyperinsulinemia.