In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung
In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung
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使用吸水链霉菌亚种抑制恶性疟原虫 3D7 寄生虫的体外试验
DOI：
10.22207/jpam.15.2.45
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发表时间：
2021
影响因子：
0.8
通讯作者：
Ulin Ni’mah Setiawati
中科院分区：
文献类型：
--
作者：
E. Setyaningrum;A. Arifiyanto;N. Nukmal;T. N. Aeny;Meishy Handerlin Putri;Ulin Ni’mah Setiawati
对天然成分的认识和利用的增加，导致对天然药用成分来源的需求增加，包括用于治疗疟疾的化合物来源。链霉菌属是一种原核生物，因其生产抗生素和其他药学上有用的化合物而被广泛认可。本研究旨在评估未纯化的发酵代谢产物抑制疟原虫寄生虫的能力。一株经鉴定为吸水链霉菌吸水亚种的细菌。从楠榜省的菠萝田中分离到吸水菌菌株i18，并在液体合成Gause培养基上培养和发酵10天。从细胞中分离上清液，用乙酸乙酯-甲醇（1：1）提取。恶性疟原虫3D 7用于抗疟原虫测试。使用植物化学方法定性地测试了代谢物。皂苷和三萜类化合物被发现存在于提取物中。使用概率单位分析测量的寄生虫抑制，产生的IC 50值为11.07 g.m/L。这些结果表明，有必要对该提取物进行进一步检查（例如，脱靶效应评估）。
Increasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off-target effects) are warranted.