Soy undecapeptide induces Drosophila hind leg grooming via dopamine receptor.
Soy undecapeptide induces Drosophila hind leg grooming via dopamine receptor.
复制标题
大豆十一肽通过多巴胺受体诱导果蝇后腿梳理。
DOI：
10.1016/j.bbrc.2018.03.162
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ohinata K.
中科院分区：
文献类型：
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作者：
Karim MR;Yanagawa A;Ohinata K.
β-伴大豆球蛋白α亚单位[βCGα(323-333)]是一种外源性神经调节十一肽，是从小鼠行为学实验中对大豆主要储藏蛋白β-伴大豆球蛋白的酶解中发现的。我们研究了βCGα(323-333)对果蝇行为的影响。口服βCGα(323-333)可增加果蝇后腿的梳理，这可能通过特定的神经元组起作用。有报道称多巴胺受体参与了后腿的梳理，我们用基因敲除的果蝇检测了多巴胺受体对βCGα(323-333)诱导的后腿梳理的影响。在野生型果蝇中，βCGα(323-333)增加了后腿的梳理，而在基因敲除的果蝇中则没有。这些结果提示，βCGα(323-333)通过激活多巴胺受体而诱导后腿梳理。这是第一个显示外源性多肽改变苍蝇行为的报告。
β-Conglycinin α subunit (323-333) [βCGα(323-333)] is an exogenous neuromodulating undecapeptide found from enzymatic digest of β-conglycinin, a soy major storage protein by mice behavior tests. We investigated effect of βCGα(323-333) onDrosophilabehavior. Oral administration of βCGα(323-333) inDrosophilaincreased hind leg grooming, which may act through specific sets of neurons. It was reported that dopamine receptor (DopR) meditates hind leg grooming, and we tested involvement ofDopRin βCGα(323-333)-induced hind leg grooming by usingDopRknockout flies. In the wild type but not in theDopR-knockout flies, βCGα(323-333) increased hind leg grooming. These results suggest that βCGα(323-333) induces hind leg grooming via activating theDopR. This is the first report showing that exogenously administered peptide changes fly behaviors.