A rapid assay for determination of antiviral activity against human cytomegalovirus
A rapid assay for determination of antiviral activity against human cytomegalovirus
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DOI：
10.1016/0166-3542(95)00976-0
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发表时间：
1996-08-01
影响因子：
7.6
通讯作者：
Dilworth, VM
中科院分区：
文献类型：
--
作者：
Hippenmeyer, PJ;Dilworth, VM
RC 256，表达β-半乳糖苷酶的重组人巨细胞病毒（HCMV），被用作快速筛选抗病毒活性化合物的工具。抑制RC 256所需的抗病毒化合物的有效浓度与抑制其它HCMV株所需的浓度相同，如通过空斑减少测定或病毒产率减少测定所测量的。测量感染细胞裂解物中的β-半乳糖苷酶活性可以确定感染后48小时的有效浓度，其结果与更长、更费力的测定法相当。
RC256, the recombinant human cytomegalovirus (HCMV) which expresses beta-galactosidase, was used as a tool for rapid screening of compounds for antiviral activity. The effective concentration of antiviral compound needed to inhibit RC256 was identical to the concentrations needed to inhibit other strains of HCMV as measured by plaque reduction assay or virus yield reduction assay. Measurement of beta-galactosidase activity in infected cell lysates allowed determination of effective concentrations 48 h postinfection with results comparable to the longer, more laborious assays.