Synergistic Effect of Micro-Nano-Hybrid Surfaces and Sr Doping on the Osteogenic and Angiogenic Capacity of Hydroxyapatite Bioceramics Scaffolds.
Synergistic Effect of Micro-Nano-Hybrid Surfaces and Sr Doping on the Osteogenic and Angiogenic Capacity of Hydroxyapatite Bioceramics Scaffolds.
复制标题
微纳复合表面和锶掺杂对羟基磷灰石生物陶瓷支架成骨及血管生成能力的协同作用
DOI：
10.2147/ijn.s345357
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发表时间：
2022
影响因子：
8
通讯作者：
Cai M
中科院分区：
文献类型：
--
作者：
Jiang S;Wang X;Ma Y;Zhou Y;Liu L;Yu F;Fang B;Lin K;Xia L;Cai M
化学元素掺杂和表面修饰的协同作用被认为是调控细胞生物学反应和提高生物材料成骨诱导能力的新途径。采用水热转化法制备了微纳米杂化（微棒和纳米棒的混合物）表面和不同锶（Srx-mnHAp, x: 2.5、5、10和20%）掺杂量的羟基磷灰石（HAp）生物陶瓷。体外评价Srx-mnHAp对骨髓基质细胞（BMSCs）成骨和血管生成的影响，并进一步将生物陶瓷支架植入大鼠颅骨缺损，观察体内骨再生情况。具有微纳杂化表面的HAp生物陶瓷（mnHAp）能够促进细胞的扩散、增殖能力、ALP活性以及成骨和血管生成因子COL1、BSP、BMP-2、OPN、VEGF和ANG-1的基因表达。更重要的是，Srx-mnHAp （x: 2.5、5、10和20%）进一步促进了细胞成骨活性，其中Sr10-mnHAp具有最好的刺激作用。颅骨缺损的实验结果显示，与mnHAp和HAp生物陶瓷（致密和平坦的表面）相比，Sr10-mnHAp可以促进更多的骨和血管再生。本研究表明，微纳杂化表面的羟基磷灰石生物陶瓷与锶掺杂具有协同促进骨再生的作用，是一种很有前途的骨缺损修复材料。
The synergistic effect of chemical element doping and surface modification is considered a novel way to regulate cell biological responses and improve the osteoinductive ability of biomaterials. Hydroxyapatite (HAp) bioceramics with micro-nano-hybrid (a mixture of microrods and nanorods) surfaces and different strontium (Sr) doping contents of 2.5, 5, 10, and 20% (Srx-mnHAp, x: 2.5, 5, 10 and 20%) were prepared via a hydrothermal transformation method. The effect of Srx-mnHAp on osteogenesis and angiogenesis of bone marrow stromal cells (BMSCs) was evaluated in vitro, and the bioceramics scaffolds were further implanted into rat calvarial defects for the observation of bone regeneration in vivo. HAp bioceramics with micro-nano-hybrid surfaces (mnHAp) could facilitate cell spreading, proliferation ability, ALP activity, and gene expression of osteogenic and angiogenic factors, including COL1, BSP, BMP-2, OPN, VEGF, and ANG-1. More importantly, Srx-mnHAp (x: 2.5, 5, 10 and 20%) further promoted cellular osteogenic activity, and Sr10-mnHAp possessed the best stimulatory effect. The results of calvarial defects revealed that Sr10-mnHAp could promote more bone and blood vessel regeneration, with mnHAp and HAp bioceramics (dense and flat surfaces) as compared. The present study suggests that HAp bioceramics with micro-nano-hybrid surface and Sr doping had synergistic promotion effects on bone regeneration, which can be a promising material for bone defect repair.