HD-ZIP I transcription factor (PtHB13) negatively regulates citrus flowering through binding to FLOWERING LOCUS C promoter
HD-ZIP I transcription factor (PtHB13) negatively regulates citrus flowering through binding to FLOWERING LOCUS C promoter
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HD-ZIP I 转录因子 (PtHB13) 通过与开花基因座 C 启动子结合来负调控柑橘开花
DOI：
10.3390/plants9010114
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Chun-Gen Hu
中科院分区：
文献类型：
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作者：
Yu-Jiao Ma;Pei-Ting Li;Lei-Ming Sun;Huan Zhou;Ren-Fang Zeng;Xiao-Yan Ai;Jin-Zhi Zhang;Chun-Gen Hu
对于成年柑橘的成花诱导，低温是最重要的环境因素之一。低温诱导拟南芥开花过程中，FLC基因的表达受到抑制，在低温诱导拟南芥开花过程中起重要作用。然而，很少有人知道FLC的调控机制，在多年生木本植物，如柑橘。本研究通过在拟南芥中异位表达的方法，对柑橘FLC同源基因（PtFLC）的功能进行了研究。通过酵母单杂交筛选，发现同源域亮氨酸拉链I转录因子（HD-ZIP I）是PtFLC的上游调控因子，对PtFLC的转录起调控作用。HD-ZIP I转录因子与拟南芥ATHB 13高度同源，因此命名为PtHB 13。在转基因拟南芥中异位表达PtHB 13抑制开花。PtFLC和PtHB 13的表达在成花诱导期表现出明显的季节性变化，并受低温的影响。因此，我们建议PtHB 13结合PtFLC启动子调节其活性在柑橘成花诱导过程中。
For floral induction in adult citrus, low temperature is one of the most important environmental factors. FLOWERING LOCUS C (FLC) plays a very important role in low-temperature-induced Arabidopsis flowering by repressed FLC expression under exposure to prolonged low-temperature conditions. However, little is known about the FLC regulation mechanism in perennial woody plants such as citrus. In this study, the functions of citrus FLC homolog (PtFLC) were investigated by ectopic expression in Arabidopsis. Transcription factor of homeodomain leucine zipper I (HD-ZIP I) as an upstream regulator of PtFLC was identified by yeast one-hybrid screen to regulate its transcription. The HD-ZIP I transcription factor was highly homologous to Arabidopsis ATHB13 and thus was named PtHB13. Ectopically expressed PtHB13 inhibited flowering in transgenic Arabidopsis. Furthermore, the expression of PtFLC and PtHB13 showed a seasonal change during the floral induction period and was also affected by low temperature. Thus, we propose that PtHB13 binds to PtFLC promoter to regulate its activity during the citrus floral induction process.