mixsep: An R-package for DNA mixture separation
mixsep: An R-package for DNA mixture separation
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DOI：
10.1016/j.fsigss.2011.09.104
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发表时间：
2011-12-01
期刊：
影响因子：
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通讯作者：
Tvedebrink, Torben
中科院分区：
文献类型：
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作者：
Tvedebrink, Torben
提出了一种基于 [2] STR 峰强度统计模型的混合物分离算法的实现 [4]。该实现可以在开源软件 R 中免费获得。图形用户界面 (GUI) 简化了数据导入、已知配置文件的规范和 STR 数据的分析 [3]。为峰强度制定的统计模型满足 STR 峰数据的固有比例，并假设对共享等位基因的贡献在 DNA 总量方面是相加的。用于分离 DNA 混合物的模型和算法经过验证，得到了与三位经验丰富的法医遗传学家相似的结果。基于模型的方法的优点是实验室内病例报告的一致性、提高的速度以及比较不同提议的 DNA 图谱组合的客观措施。此外，统计模型可用于计算预期峰值强度，将其与观察到的峰值强度绘制在一起，为传达分析结论提供了强大的工具。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
An implementation of a mixture separating algorithm based on a statistical model for [2] STR peak intensities is presented [4]. The implementation is freely available in the open source software R. A graphical user interface (GUI) eases the data importing, specification of known profiles and analysis of the STR data [3].The statistical model formulated for the peak intensities satisfy intrinsic proportions of the STR peak data, and assumes that the contribution to shared alleles is additive in terms of the total amount of DNA. The model and algorithm for separating DNA mixtures was validated and gave similar results as those of three experienced forensic geneticists. The advantages of the model-based approach are consistency in case reports within a laboratory, increased speed and objective measures for comparing different proposed combinations of DNA profiles. Furthermore, the statistical model can be used to compute expected peak intensities, which plotted together with the observed peak intensities provide a powerful tool for communicating the conclusions of an analysis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.