Changes in the morphology and functions of vacuoles during the death of ray parenchyma cells in Cryptomeria japonica
Changes in the morphology and functions of vacuoles during the death of ray parenchyma cells in Cryptomeria japonica
复制标题
DOI：
10.1007/s10086-017-1692-6
复制
发表时间：
2018-06-01
影响因子：
2.9
通讯作者：
Nakaba, Satoshi
中科院分区：
文献类型：
--
作者：
Arakawa, Izumi;Funada, Ryo;Nakaba, Satoshi
空泡形态和功能的变化为细胞死亡机制提供了有用的信息。本研究对柳杉射线薄壁细胞死亡过程中液泡的形态和含量进行了动态观察。在木质部的分化过程中，射线薄壁细胞具有大的中央液泡。边材射线薄壁细胞中的液泡含有蛋白质，这表明这些液泡的主要功能之一可能是储存蛋白质。在液泡破裂开始之前，在中间木材中检测到一些液泡的蛋白质含量急剧下降。从中间材到最外层心材，空泡破裂，但在中间材内部，有些空泡明显扩大。射线薄壁细胞中的大液泡破裂后，首先观察到浓缩的细胞核。射线薄壁组织细胞内含物的自溶可能是由于内部中间材中扩大的液泡破裂所致。
Changes in the morphology and functions of vacuoles provide useful information about the mechanism of cell death. In the present study, we monitored the morphology and contents of vacuoles during the death of ray parenchyma cells in the conifer Cryptomeria japonica. In differentiating xylem, ray parenchyma cells had large central vacuoles. In sapwood, vacuoles in ray parenchyma cells contained proteins, an indication that one of the main functions of these vacuoles might be protein storage. A dramatic decrease in the protein content of some vacuoles was detected in the intermediate wood before the initiation of vacuole rupture. Although vacuole rupture was detected from the intermediate wood to the outermost heartwood, some vacuoles were obviously enlarged in the inner intermediate wood. Condensed nuclei were first observed after the rupture of these large vacuoles in ray parenchyma cells. It seems plausible that the autolysis of the contents of ray parenchyma cells might be caused by the rupture of the enlarged vacuoles in the inner intermediate wood.