Genomic Comparison of Insect Gut Symbionts from Divergent Burkholderia Subclades
Genomic Comparison of Insect Gut Symbionts from Divergent Burkholderia Subclades
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DOI：
10.3390/genes11070744
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发表时间：
2020-07-01
期刊：
影响因子：
3.5
通讯作者：
Kikuchi, Yoshitomo
中科院分区：
文献类型：
--
作者：
Takeshita, Kazutaka;Kikuchi, Yoshitomo
蝽总科Coreoidea和Lygaeoidea的臭虫与环境中获得的Burkholderiasensu lato属细菌建立肠道共生关系。在伯克霍尔德氏菌属中，与臭虫相关的菌株形成一个单系分支，称为臭虫相关有益环境（SBE）分支（或Caballeronia）。最近，我们发现Pyrrhocoroidea总科中的Largidae科成员与Burkholderia相关，但不是特别与SBE Burkholderia相关; largid bugs的共生体属于Burkholderia植物相关组的一个分支，称为植物相关有益和环境（PBE）分支（或Paraburkholderia）。为了了解蝽类伯克霍尔德氏菌的基因组特征，我们从一种有缘植物蝽Physopellta gutta（Pyrrhocoroidea：Largidae）中分离了两株共生伯克霍尔德氏菌，并测定了它们的完整基因组。昆虫相关PBE（iPBE）的基因组大小为9.5 Mb和11.2 Mb，两者都大于SBE Burkholderiasymbionts的基因组。两种iPBE共生体和三种SBE共生体之间的全基因组比较强调，所有先前报道的共生因子都是共享的，并且在五种臭虫共生体中有282个基因是特别保守的，其中超过三分之一的功能未知。在共生体特异性基因中，大约40个基因在所有五种共生体中形成一个簇;这表明与臭虫相关的伯克霍尔德氏菌基因组中存在一个“共生岛”。
Stink bugs of the superfamilies Coreoidea and Lygaeoidea establish gut symbioses with environmentally acquired bacteria of the genusBurkholderiasensu lato. In the genusBurkholderia, the stink bug-associated strains form a monophyletic clade, named stink bug-associated beneficial and environmental (SBE) clade (orCaballeronia). Recently, we revealed that members of the family Largidae of the superfamily Pyrrhocoroidea are associated withBurkholderiabut not specifically with the SBEBurkholderia; largid bugs harbor symbionts that belong to a clade of plant-associated group ofBurkholderia, called plant-associated beneficial and environmental (PBE) clade (orParaburkholderia). To understand the genomic features ofBurkholderiasymbionts of stink bugs, we isolated two symbioticBurkholderiastrains from a bordered plant bugPhysopellta gutta(Pyrrhocoroidea: Largidae) and determined their complete genomes. The genome sizes of the insect-associated PBE (iPBE) are 9.5 Mb and 11.2 Mb, both of which are larger than the genomes of the SBEBurkholderiasymbionts. A whole-genome comparison between two iPBE symbionts and three SBE symbionts highlighted that all previously reported symbiosis factors are shared and that 282 genes are specifically conserved in the five stink bug symbionts, over one-third of which have unknown function. Among the symbiont-specific genes, about 40 genes formed a cluster in all five symbionts; this suggests a "symbiotic island" in the genome of stink bug-associatedBurkholderia.