Development in vitro and in vivo of aggregated parthenogenetic bovine embryos.
Development in vitro and in vivo of aggregated parthenogenetic bovine embryos.
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聚集的孤雌牛胚胎的体外和体内发育。
DOI：
10.1071/rd9951073
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
T. Suzuki
中科院分区：
文献类型：
--
作者：
A. Boediono;S. Saha;C. Sumantri;T. Suzuki
成熟牛卵母细胞用7%乙醇激活，然后用细胞松弛素B或D处理。大多数卵母细胞挤出第二极体，形成一个原核时，用7%的乙醇单独处理[35/43（81%）]。用乙醇，然后用细胞松弛素B或D，总的激活频率为70%（309/441），激活的卵母细胞含有两个原核。单用乙醇和乙醇+细胞松弛素B处理的卵裂率无显著差异，但显著低于受精卵（P < 0.01）。但囊胚发育率在各处理组间差异极显著（P < 0.01）。孤雌胚胎的染色体异常率为32%（8/25），正常（二倍体）和染色体异常（2N/4 N）的孤雌胚胎的发生率分别为68%（17/25）和32%（8/25），且不受冷冻处理的影响。体外培养的4枚聚集胚的纵径显著大于2枚或1枚聚集胚（P < 0.01）。将聚集的四个孤雌生殖胚胎中的一个进一步体外培养，并在激活后发育至第27天，直径为2980微米。将聚集的4枚孤雌胚移植给5只受体。3只受体发情期延长，分别于发情后第57、62和67天恢复发情。这些结果表明，聚集孤雌胚胎可以延长其在体外和体内的存活时间。
Mature bovine oocytes were activated with 7% ethanol followed by cytochalasin B or D treatment. Most oocytes extruded a second polar body and formed one pronucleus when treated with 7% ethanol alone [35/43 (81%)]. With ethanol followed by cytochalasin B or D, overall activation frequency was 70% (309/441), with activated oocytes containing two pronuclei. The cleavage rate was not significantly different between treatment with ethanol alone and ethanol followed by 5 micrograms mL-1 cytochalasin B, but it was significantly lower than in fertilized oocytes (P < 0.01). However, the blastocyst production rate was significantly different (P < 0.01) among the treatments. The incidence of parthenogenetic embryos with normal (diploid) complements and with chromosome anomalies (2N/4N) was 68% (17/25) and 32% (8/25) respectively, and this was not affected by cryopreservation treatment. The longitudinal diameter of aggregated-four embryos cultured in vitro was greater (P < 0.01) than aggregated-two or single embryos. One of the aggregated-four parthenogenetic embryos was further cultured in vitro and developed up to Day 27 after activation, with a diameter of 2980 microns. The aggregated-four parthenogenetic embryos were transferred to five recipients. The oestrus was prolonged in three recipients and they returned to oestrus on Day 57, 62 and 67 after the previous oestrus. These results indicate that aggregating parthenogenetic embryos can prolong their survival in vitro and in vivo.