Raman spectroscopic investigation on the molecular structure of apatite and collagen in osteoporotic cortical bone
Raman spectroscopic investigation on the molecular structure of apatite and collagen in osteoporotic cortical bone
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DOI：
10.1016/j.jmbbm.2016.08.030
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发表时间：
2017-01-01
影响因子：
3.9
通讯作者：
Valdre, Giovanni
中科院分区：
文献类型：
--
作者：
Pezzotti, Giuseppe;Rondinella, Alfredo;Valdre, Giovanni
本研究采用高光谱分辨的拉曼光谱来检查从健康和卵巢切除术（OVX）诱导的骨质疏松状态的小鼠雌性获得的皮质骨组织的分子组成。本研究的目的是确定两组样品之间磷灰石矿物和胶原纤维在分子尺度上的结构差异。拉曼光谱用于确定包括骨矿物质和骨基质的特征带的区域中的皮质骨的化学组成。结果表明，骨中的矿物磷灰石在其患病状态下没有发生显著的非晶化，拉曼微探针也未能识别碳酸盐含量在OVX患病骨脆化中的直接作用。另一方面，根据拉曼谱带的形态学变化，在骨羟基磷灰石的柱状Ca结构中可以检测到复杂的非化学计量变化，该谱带属于对称磷酸盐伸缩（A（1））谱带，在959 cm（-1）附近。胶原蛋白质量在骨脆化过程中也起着重要作用。在酰胺I区域的拉曼光谱上系统测量的所谓基质成熟度比，与对照样品相比，在OVX处理的样品中具有统计学显著性。与对照组相比，8%的增加可能与OVX患病组织矿物质相中弹性应力增强增加115%相关，从而证明患病骨基质的（弹性）能量耗散能力下降。(C)2016爱思唯尔有限公司版权所有。
This study employed highly spectrally resolved Raman spectroscopy to examine the molecular composition of cortical bone tissue obtained from murine females in their healthy and ovariectomy- (OVX-) induced osteoporotic states. The aim of the study was to identify structural differences at the molecular scale both in apatite mineral and collagen fibrils between the two groups of samples. Raman spectroscopy was used to determine the chemical composition of cortical bone in regions including characteristic bands of both bone mineral and bone matrix. The results demonstrated that the mineral apatite of bone did not undergo significant amorphization in its diseased state, with the Raman microprobe also failing in recognizing a direct role of carbonate content in the embrittlement of OVX-diseased bone. On the other hand, complex off-stoichiometry variations could be detected in the columnar Ca-structure of the bony hydroxyapatite according to morphological variations of the Raman band belonging to the symmetric phosphate stretching (A(1)) band at similar to 959 cm(-1). A fundamental role was also recognized for collagen quality on the process of bone embrittlement. The so-called matrix maturity ratio, as systematically measured on Raman spectra in the Amide I region, increased with statistical significance in OVX-treated samples as compared to control samples. An 8% increase could be associated to a 115% increase in elastic stress intensification in the mineral phase of OVX-diseased tissue as compared to the control one, thus proving a degradation in the (elastic) energy-dissipative capacity of a diseased bone matrix. (C) 2016 Elsevier Ltd. All rights reserved.