Chemical Functionalization of Polysilicon Microparticles for Single-Cell Studies
Chemical Functionalization of Polysilicon Microparticles for Single-Cell Studies
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DOI：
10.1021/la200857x
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发表时间：
2011-07-05
期刊：
影响因子：
3.9
通讯作者：
Fernandez-Sanchez, C.
中科院分区：
文献类型：
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作者：
Fernandez-Rosas, E.;Baldi, A.;Fernandez-Sanchez, C.
在这项工作中，用特定的配体修饰了两种类型的多晶硅（多晶硅）微粒，以便选择性地附着在质膜上的化学残基上，从而应用于培养中的单个细胞的研究。研究了基于吸附和共价附着的两种不同功能化方法。用荧光团标记所选择的配体，对比研究了配体固定的效率和修饰颗粒在培养基中的稳定性。使用圆柱形微粒子（非编码微粒子）和形状编码微粒子（条形码），目的是证明颗粒大小和形状对固定方案效率的不依赖性。荧光成像和记录的荧光强度统计分析表明，配体与微粒表面的共价附着，先前用端醛硅烷修饰，给出了最好的结果。作为概念的证明，用共价修饰的条形码标记培养中的Vero细胞，并成功地跟踪了长达1周，未观察到细胞活力的任何变化。使用明场光学显微镜可以很容易地用眼睛读取条形码。预计这种修饰的微粒可以作为引入其他分析功能的可行平台，用于自动分析系统中的单细胞监测和细胞分选。
In this work, two types of polycrystalline silicon (polysilicon) microparticles were modified with specific ligands in order to be selectively attached to chemical residues located at the plasma membrane and thus to be applied to study individual cells in culture. Two different functionalization approaches based on adsorption and covalent attachment were assayed. A comparative study of the efficiency of the ligand immobilization and stability of the modified particle in the culture medium was carried out using the selected ligands labeled with a fluorophore. Cylindrical microparticles (nonencoded microparticles) and shape-encoded micropartcles (bar codes) were used with the aim of demonstrating the nondependence of the particle size and shape on the efficiency of the immobilization protocol. Fluorescence imaging and statistical analysis of the recorded fluorescence intensity showed that the covalent attachment of the ligand to the surface of the microparticle, previously modified with an aldehyde-terminated silane, gave the best results. As a proof of concept, Vero cells in culture were labeled with the covalently modified bar codes and successfully tracked for up to 1 week without observing any alteration in the viability of the cells. Bar code numbers could be easily read by eye using a bright-field optical microscope. It is anticipated that such modified microparticles could be feasible platforms for the introduction of other analytical functions of interest in single-cell monitoring and cell sorting in automatic analysis systems.