Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy
Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy
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滤泡树突状细胞分泌蛋白表达的降低与免疫球蛋白 A 肾病患者扁桃体中免疫球蛋白 A 的产生增加相关
DOI：
10.1016/j.trsl.2015.04.004
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发表时间：
2015-09-01
影响因子：
7.8
通讯作者：
Yamakawa, Mitsunori
中科院分区：
文献类型：
--
作者：
Meng, Hong-Xue;Li, Hui-Ning;Yamakawa, Mitsunori
免疫球蛋白A肾病（IgAN）的特征是伊加在循环中的定性异常和伊加在肾系膜中的沉积。最近的研究表明，致病性伊加可能来源于受影响的扁桃体。滤泡树突状细胞分泌蛋白（FDC-SP）是一种新型的分泌性蛋白，可调节B细胞反应的诱导，并被认为可控制伊加的产生。在此背景下，本研究调查了IgAN患者扁桃体中FDC-SP的表达及其与伊加产生的相关性。应用免疫组织化学和逆转录聚合酶链反应（RT-PCR）方法比较IgAN伴扁桃体炎患者和非IgAN伴慢性扁桃体炎患者扁桃体中FDC-SP的表达。FDC-SP在扁桃体组织中的定位通过双重免疫荧光证实。我们发现FDC-SP的表达显着下降，并与伊加产生的IgAN患者的扁桃体增强呈负相关。滤泡树突状细胞分泌的FDC-SP可能作用于生发中心B细胞，参与调节扁桃体伊加的生成。我们的研究表明，FDC-SP可能参与伊加产生的IgAN患者的扁桃体，使这种蛋白质一个有吸引力的候选免疫调节剂，并突出了一个有前途的策略，在IgAN的治疗干预。
Immunoglobulin A nephropathy (IgAN) is characterized by a qualitative abnormality of IgA in the circulation and IgA deposition in the renal mesangium. Recent research has indicated that pathogenic IgA may originate from affected tonsils. Follicular dendritic cell-secreted protein (FDC-SP), a small novel secretory protein that may regulate the induction of B-cell responses, has been suggested to control IgA production. Given this background, this study investigated the expression of FDC-SP and its correlation with IgA production in the tonsils of IgAN patients. lmmunohisto-chemistry and reverse transcription-polymerase chain reaction were used to compare the expression of FDC-SP in the tonsils of IgAN patients with tonsillitis and of non-IgAN patients with chronic tonsillitis. The location of FDC-SP in tonsillar tissue was confirmed by double immunofluorescence. We found that FDC-SP expression significantly decreased and was correlated negatively with enhanced IgA production in the tonsils of IgAN patients. FDC-SP secreted by follicular dendritic cells may act on germinal center B cells and participate in the modulation of IgA generation in the tonsils. Our study demonstrated that FDC-SP may be involved in IgA production in the tonsils of IgAN patients, making this protein an attractive candidate immunomodulator, and highlighting a promising strategy for therapeutic intervention in IgAN.