In vitro susceptibility of Gabonese wild isolates of Plasmodium falciparum to artemether, and comparison with chloroquine, quinine, halofantrine and amodiaquine
In vitro susceptibility of Gabonese wild isolates of Plasmodium falciparum to artemether, and comparison with chloroquine, quinine, halofantrine and amodiaquine
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DOI：
10.1017/s0031182098003400
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发表时间：
1998-12-01
期刊：
影响因子：
2.4
通讯作者：
Kombila, M
中科院分区：
文献类型：
--
作者：
Pradines, B;Mamfoumbi, MM;Kombila, M
使用同位素药物敏感性半微量试验评价了蒿甲醚对来自加蓬利伯维尔的63株非洲恶性疟原虫分离株的体外活性。蒿甲醚的50%抑制浓度（IC 50）值在0.8 - 34.8 nM的窄范围内（平均IC 50 5.0 nM），95%置信区间（CI 95%）为3.6-6.3 nM。在体外观察到蒿甲醚（14%）、氯喹（90%）和奎宁（32%）的敏感性或耐药性降低。分离株对阿莫地喹和卤泛群的敏感性较高，分别为100%和98%.蒿甲醚与阿莫地喹（r（2）= 0.45，P < 0.001）、蒿甲醚与氯喹（r（2）= 0.36，P < 0.001）、蒿甲醚与奎宁（r（2）= 0.31，P < 0.001）、蒿甲醚与卤泛群（r（2）= 0.19，P < 0.01）的反应之间存在显著正相关。这些药物之间的正相关性表明体外交叉耐药性或至少是药物摄取和/或作用模式或耐药性的共同特征。
The in vitro activity of artemether against 63 African isolates of Plasmodium falciparum from Libreville, Gabon was evaluated using an isotopic drug susceptibility semi-microtest. The 50 % inhibitory concentration (IC50) values for artemether were in a narrow range from 0.8 to 34.8 nM (mean IC50 5.0 nM) and the 95 % confidence interval (CI95 %) was 3.6-6.3 nM. In vitro decreased susceptibility or resistance were observed with artemether (14 %), to chloroquine (90 %), to quinine (32 %). Isolate susceptibility to amodiaquine and halofantrine was high i.e. 100 % and 98 %, respectively. There was a significant positive correlation between responses to artemether and amodiaquine (r(2) = 0.45, P < 0.001), artemether and chloroquine (r(2) = 0.36, P < 0.001), artemether and quinine (r(2) = 0.31, P < 0.001), and artemether and halofantrine (r(2) = 0.19, P < 0.01). Positive correlation between these drugs suggests in vitro cross-resistance or at least common features in drug uptake and/or mode of action or resistance.