Seeing the Invisible: Revealing Atrial Ablation Lesions Using Hyperspectral Imaging Approach.
Seeing the Invisible: Revealing Atrial Ablation Lesions Using Hyperspectral Imaging Approach.
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DOI：
10.1371/journal.pone.0167760
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sarvazyan NA
中科院分区：
文献类型：
--
作者：
Muselimyan N;Swift LM;Asfour H;Chahbazian T;Mazhari R;Mercader MA;Sarvazyan NA
目前，在射频消融过程中高分辨率监测组织损伤的手段有限。开发下一代可视化导管，可以揭示消融引起的不可逆心房肌损伤，并识别病变之间的生存能力差距。将射频损伤放置在离体的人和牛心房的心内膜表面以及血液灌注的大鼠心脏的左心室上。用 365 nm 光照射组织，并从 420-720 nm 范围内的各个光谱带获取一系列图像。通过提取单个像素的光谱轮廓和光谱分解，然后显示烧蚀和未烧蚀光谱对每个像素的相对贡献。将光谱分离的结果与病变病理学进行比较。射频消融引起组织自发荧光轮廓的显着变化。人类左心房中这些光谱变化的幅度相对较小（<荧光峰值值的 10%），但非常显着。高光谱数据集的光谱分解实现了高空间分辨率，无需外源标记即可原位描绘射频病变边界。来自高光谱成像方法的病变尺寸与组织学结果密切相关。心肌内血液的存在降低了自发荧光光谱的幅度，同时对其整体形状的影响最小。因此，高光谱成像描绘体内消融病变的能力没有受到影响。高光谱成像极大地增加了消融和未消融组织之间的对比度，从而能够可视化临床相关位置的活力差距。数据支持开发用于高分辨率消融引导的经皮高光谱导管的可能性。
Currently, there are limited means for high-resolution monitoring of tissue injury during radiofrequency ablation procedures. To develop the next generation of visualization catheters that can reveal irreversible atrial muscle damage caused by ablation and identify viability gaps between the lesions. Radiofrequency lesions were placed on the endocardial surfaces of excised human and bovine atria and left ventricles of blood perfused rat hearts. Tissue was illuminated with 365nm light and a series of images were acquired from individual spectral bands within 420-720nm range. By extracting spectral profiles of individual pixels and spectral unmixing, the relative contribution of ablated and unablated spectra to each pixel was then displayed. Results of spectral unmixing were compared to lesion pathology. RF ablation caused significant changes in the tissue autofluorescence profile. The magnitude of these spectral changes in human left atrium was relatively small (< 10% of peak fluorescence value), yet highly significant. Spectral unmixing of hyperspectral datasets enabled high spatial resolution, in-situ delineation of radiofrequency lesion boundaries without the need for exogenous markers. Lesion dimensions derived from hyperspectral imaging approach strongly correlated with histological outcomes. Presence of blood within the myocardium decreased the amplitude of the autofluorescence spectra while having minimal effect on their overall shapes. As a result, the ability of hyperspectral imaging to delineate ablation lesions in vivo was not affected. Hyperspectral imaging greatly increases the contrast between ablated and unablated tissue enabling visualization of viability gaps at clinically relevant locations. Data supports the possibility for developing percutaneous hyperspectral catheters for high-resolution ablation guidance.
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DOI：
10.1161/circep.114.001750
发表时间：
2014-10
期刊：
Circulation. Arrhythmia and electrophysiology
影响因子：
--
作者：
Swift L;Gil DA;Jaimes R 3rd;Kay M;Mercader M;Sarvazyan N
通讯作者：
Sarvazyan N
DOI：
10.1117/12.2043796
发表时间：
2014-03-21
期刊：
Proceedings of SPIE--the International Society for Optical Engineering
影响因子：
--
作者：
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通讯作者：
Fei B
DOI：
10.1111/j.1540-8167.2000.tb00068.x
发表时间：
2000-08-01
影响因子：
2.7
作者：
Saito, T;Waki, K;Becker, AE
通讯作者：
Becker, AE
影响因子：
1.9
作者：
PICKERING, JW;BOSMAN, S;VANGEMERT, MJC
通讯作者：
VANGEMERT, MJC
影响因子：
3.4
作者：
Baxter, WT;Mironov, SF;Pertsov, AM
通讯作者：
Pertsov, AM